多肉植物筒叶花月离体再生技术探究  

Study on the Culture and Propagation of Crassula obliqua Gollum

作　　者：刘艳妮[1] 高露 王飞[2] Liu Yanni;Gao Lu;Wang Fei(Yinchuan University of Energy, Yongning,750105;College of Horticulture, Northwest A&F University, Yangling,712100)

机构地区：[1]银川能源学院生物工程系,永宁750105 [2]西北农林科技大学园艺学院,杨陵712100

出　　处：《分子植物育种》2018年第23期7781-7787,共7页Molecular Plant Breeding

基　　金：银川能源学院校级科研项目(2016-KY-Y-14)资助

摘　　要：为了建立筒叶花月离体快繁技术体系,进行商业化生产。本研究以筒叶花月叶片为外植体材料,对其进行诱导、分化、增殖、生根培养。结果表明,采用叶片平放方式,培养基为MS+KT1mg/L+6-BA1.2mg/L+NAA1.5mg/L的浓度配比,适合筒叶花月叶片愈伤组织的诱导;采用培养基为MS+KT1mg/L+6-BA0.6mg/L+NAA0.5mg/L的浓度配比,适合筒叶花月不定芽的分化培养;培养基为MS+6-BA1mg/L+NAA0.05mg/L的配比,适合筒叶花月不定芽的增殖。培养基为MS+KT1mg/L+6-BA0.6mg/L+NAA1mg/L的配比,适合筒叶花月不定根的诱导培养。In order to establish a rapid propagation system for Crassula obliqua Gollum in vitro and on the purpose of commercial production. In this study, the leaves of Crassula obliqua Gollum were selected as explant material to induce, proliferate, differentiate and culture rooting in vitro. The results showed that: A highly efficient regeneration system of Crassula obliqua Gollum was initially established. The optimal hormone combinations for adventitia ous bud initiation were MS medium with KT 1 mg/L, 6-BA 1.2 mg/L and NAA 0.5 mg/L, and the leaf position was horizontal onto the medium. The hormone combinations of MS medium with KT 1 mg/L, 6-BA 1.2 mg/L and NAA 2 mg/L was beneficial for callus differentiation, and the leaf position was vertical onto the medium, respectively. MS mediu with KT 0 mg/L, 6-BA 1 mg/L and NAA 0.05 mg/L was used as adventitious bud increment medium. MS mediu with KT 1 mg/L, 6-BA 0.6 mg/L and NAA 1 mg/L can be used as rooting medium.

关 键 词：筒叶花月 诱导培养 分化培养 增殖培养 生根培养 

分 类 号：S682.33[农业科学—观赏园艺]