冬虫夏草菌粉对糖尿病大鼠对比剂肾损伤的影响  

Effect of Cordyceps sinensis powder on contrast-induced nephropathy in diabetic rats

作　　者：许蔚 赵凯[1] 高巧营[3] 宗春辉[3] 高晟[1] XU Wei;ZHAO Kai;GAO Qiao-ying(Department of Cardiology,Tianjin Nankai Hospital;Graduate School of Tianjin University of Traditional Chinese Medicine;Institute of Acute Abdomen of integration of Chinese medicine and Western medicine,Tianjin 300100,China)

机构地区：[1]天津市南开医院心内一科,天津300100 [2]天津中医药大学研究生院,天津300193 [3]天津市南开医院中西医结合急腹症研究所,天津300100

出　　处：《中国实验诊断学》2018年第12期2136-2140,共5页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金项目(81503409)

摘　　要：目的观察冬虫夏草菌粉对糖尿病大鼠对比剂肾病的影响,探讨其可能的肾保护机制。方法将100只雄性SD大鼠腹腔注射链脲佐菌素(60mg/kg)制备成糖尿病模型,造模成功大鼠84只,随机分为对照组(N组),对比剂肾病组(M组),冬虫夏草干预组(C组),普罗布考干预组(P组,阳性对照组)4组。糖尿病造模饲养9周后,C组大鼠给予冬虫夏草菌粉(3g/kg/d)灌胃,P组给予普罗布考(500mg/kg)灌胃,其余各组给予等量的生理盐水灌胃,连续7天;第8天经尾静脉注射碘克沙醇注射液(2.0g iodine/kg)制备CIN模型,于注射对比剂前及注射24h后,血标本检测Scr、BUN;尿标本检测NGAL、KIM-1。开腹后采集双侧肾脏,取左肾用于病理;右肾用于Western blotting检测抗凋亡蛋白和促凋亡蛋白在肾内的表达量变化水平。结果(1)与M组相比,C、P组BUN、Scr均明显减低(P均<0.05)。(2)与M组比较,C、P组肾脏Bax、caspase-3表达水平降低,Bcl-2表达升高(P均<0.05)。结论冬虫夏草可减轻糖尿病大鼠CIN的早期肾脏损伤,其机制可能与下调Bax、caspase-3表达,上调Bcl-2/Bax比值,抗细胞凋亡有关,且效果与普罗布考相当。Objective Objective To explore the effect of Cordyceps sinensis powder on contrast-induced nephropathy (CIN)in diabetic rats,and investigate the mechanism in prevention of CIN in diabetic rats.Methods Totally 100rats were selected to make the models of diabetic rats which were established by abdominal single dose injection STZ 60 mg/ kg.The 84 successful established diabetic rat models were randomly divided into 4 groups,the control group (N group), the model of CIN group (M group),cordyceps sinensis group (C group),and probucol group (group P,positive con- trol).After 9-week feeding,rats in the C groups receieved intragastric administration of Cordyceps sinensis powder [3 g/(kg,d)]in 7 days,rats in the P groups receieved probucol (500 mg/kg),the other groups were perfused with normal saline of equal volume.On the eighth day,make the models of CIN which were established by intravenous injection of iodixanol (2.0 g iodine/kg)via tail vein.Before injection and after 24 h,blood samples were taken to measure Scr, BUN,urine samples were taken to measure NGAL,KIM-1;the left kidney was used for pathological indicators;the right kidney was used for Western blotting to detect the expression levels of anti apoptotic proteins and pro apoptotic proteins.Results Compared with the M group,the levels of Scr,BUN,NGALandKIM-I in the treatment group were significantly decreased(P<0.05),as well as the levels in C group and P group.According to the sequence of N group, M group,C group,P group,Western Blot showed the expression of anti apoptosis protein Bcl-2 and Bcl-2/Bax were in- creased,and the pro apoptotic protein Bax,caspase-3expression decreased.Conclusion Cordyceps sinensis powder can alleviate the renal injury of CIN in diabetic rats.The mechanism may be related to the up-regulation of Bcl-2/Bax,and down-regulation of Bax and caspase-3,in order to inhibits apoptosis.Furthermore effect in cordyceps sinensis groups is equivalent to probueol application.

关 键 词：冬虫夏草 对比剂肾病 糖尿病大鼠 细胞凋亡 

分 类 号：R587[医药卫生—内分泌]