HPLC法同时测定老蔻丸中8个成分的含量  

Simultaneous determination of eight constituents in Laokou pills by HPLC

作　　者：崔庆德 业艳芬 吕晋 CUI Qing-de;YE Yan-fen;LU Jin(Qujing Institute for Food and Drug Control,Qujing 655000,China;The First People's Hospital of Qujing,Qujing 655000,China)

机构地区：[1]曲靖市食品药品检验检测中心,曲靖655000 [2]曲靖市第一人民医院,曲靖655000

出　　处：《药物分析杂志》2018年第12期2180-2187,共8页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立HPLC法同时测定老蔻丸(陈皮、肉桂、丁香等)中8个成分的含量。方法:药物的甲醇提取液的采用依利特SinoChrom ODS-BP C_(18)色谱柱(200 mm×4.6 mm,5μm);流动相为乙腈-水-甲醇,梯度洗脱;流速为1.0 mL·min^(-1),柱温为30℃,检测波长为280 nm,进样量为15μL。结果:柚皮苷、橙皮苷、新橙皮苷、桂皮醛、丁香酚、和厚朴酚、厚朴酚、大黄酚质量浓度分别在26.669～400.031、26.803～402.042、13.335～200.028、2.566～38.484、4.272～64.086、19.994～299.908、26.682～400.223、1.068～16.014范围内与各自峰面积积分值呈良好的线性关系,相关系数r值均大于0.999;平均加样回收率(n=6)分别为100.7%、99.9%、100.1%、100.9%、99.9%、99.9%、100.6%、100.4%,RSD分别为0.77%、0.65%、0.55%、1.3%、1.4%、0.70%、0.56%、1.3%,3批样品中柚皮苷、橙皮苷、新橙皮苷、桂皮醛、丁香酚、和厚朴酚、厚朴酚、大黄酚的含量分别为1 487.42～1 678.33、6 203.50～6 982.31、1 272.29～1 410.19、236.49～292.03、1 869.33～1 896.70、3 767.62～4 073.41、2 879.84～3 250.23、279.55～301.75μg·g^(-1)。结论:该方法灵敏度高,专属性好,可用于老蔻丸的质量控制。Objective:To establish an HPLC method for the simultaneous determination of eight constituents in Laokou pills(Citri Reticulatae Pericarpium,Cinnamomi Cortex and Caryophylli Flos,etc.)Methods:The analysis of methanol extract of this drug was carried out on an Elite Sino Chrom ODS-BP C18(200 mm×4.6 mm,5 μm) column at 30 ℃,with the mobile phase comprising of acetonitrile-water-methanol at a flow rate of 1.0 mL·min^-1 in a gradient elution. And the detection wavelength was set at 280 nm. The injection volume was 15 μL. Results: Naringin,hesperidin,neohesperidin,cinnamaldehyde,eugenol,honokiol,magnolol and chrysophanol showed good linear relationships within the ranges of 26.669-400.031,26.803-402.042,13.335-200.028,2.566-38.484, 4.272-64.086,19.994-299.908,26.682-400.223 and 1.068-16.014 μg·mL^-1,respectively with correlation coefficients above 0.999. And the average recoveries(n=6)were 100.7%,99.9%,100.1%,100.9%,99.9%,99.9%, 100.6% and 100.4%,with the RSDs of 0.77%,0.65%,0.55%,1.3%,1.4%,0.70%,0.56% and 1.3%,respectively. The contents of naringin,hesperidin,neohesperidin,cinnamaldehyde,eugenol,honokiol,magnolol and chrysophanol in three batches of samples were 1 487.42-1 678.33,6 203.50-6 982.31,1 272.29-1 410.19,236.49-292.03,1 869.33- 1 896.70,3 767.62-4 073.41,2 879.84-3 250.23 and 279.55-301.75 μg·g^-1,respectively. Conclusion:The method has high sensitivity and good specificity,and can be used for the quality control of Laokou pills.

关 键 词：高效液相色谱 柚皮苷 橙皮苷 新橙皮苷 桂皮醛 丁香酚 和厚朴酚 厚朴酚 大黄酚 单波长同时测定 

分 类 号：R917[医药卫生—药物分析学]