双黄连注射液对Ⅰ型超敏反应性炎症的抑制作用  

Inhibitory effect of Shuanghuanglian Injection on inflammatory response in type Ⅰ hypersensitivity

作　　者：方蕾 费巧玲[2] 侯睿[2] 高源[2] 韩宜芯[2] 齐睿娟 蔡润兰 周鸿[3] 齐云 FANG Lei;FEI Qiao-ling;HOU Rui;GAO Yuan;HAN Yi-xin;QI Rui-juan;CAI Run-lan;ZHOU Hong;QI Yun(Department of Pharmacy,College of Medicine,Yangzhou University,Yangzhou 225009,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences &Peking Union Medical College,Beijing 100193,China;Gerontology Center of Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China)

机构地区：[1]扬州大学医学院药学系,江苏扬州225009 [2]中国医学科学院北京协和医学院药用植物研究所,北京100193 [3]中国中医科学研究院西苑医院老年病中心,北京100091

出　　处：《中国药理学与毒理学杂志》2018年第8期629-635,共7页Chinese Journal of Pharmacology and Toxicology

基　　金：国家科技重大专项(2015ZX09501004-001-003);北京市自然科学基金(7142112);江苏省高校自然科学研究面上项目(16KJB350006)~~

摘　　要：目的探讨双黄连注射液(SHLI)对Ⅰ型超敏反应性炎症的作用。方法以抗虾原肌球蛋白(ST)多抗血清致敏大鼠嗜碱性粒细胞性白血病细胞RBL-2H3,次日加入SHLI 0.5%～2.0%孵育30 min,再以ST攻击诱导其脱颗粒,荧光法检测上清β-氨基己糖苷酶含量;以佛波酯(PMA)/A23187刺激人外周血嗜碱性白血病细胞KU812,用ELISA测定其分泌肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)和前列腺素E2(PGE2)等炎症介质的水平;建立抗ST多抗血清和ST诱导的小鼠Ⅰ型超敏反应足肿胀模型,观察ip给予0.5%,1.0%和2.0%SHLI 3 d对ST攻击后1 h(速发相)和24 h(迟发相)小鼠足容积的影响。结果与模型组比较,0.5%,1.0%和2.0%SHLI均明显抑制ST所致RBL-2H3细胞β-氨基己糖苷酶的释放,荧光值分别下降16%,31%和51%(P<0.01),并显著减少PMA/A23187诱导的KU812细胞上清中炎症介质TNF-α,IL-6和PGE2的含量,TNF-α分别降低35%,55%和69%(P<0.01),IL-6分别降低29%,51%和74%(P<0.01),PGE2分别降低31%,40%和55%(P<0.01)。体内实验结果显示,速发相时SHLI 2.5和5.0 mL·kg-1使足容积差分别降低69%和83%(P<0.01),迟发相时分别降低70%和100%(P<0.05,P<0.01)。结论 SHLI可抑制Ⅰ型超敏反应性炎症。OBJECTIVE To investigate the effect of Shuanghuanglian Injection(SHLI) on the inflammatory response in type Ⅰ hypersensitivity.METHODS RBL-2 H3 cells were sensitized by anti-shrimp tropomyosin(ST) serum.Twenty-four hours later,SHLI 0.5%-2.0% was added and incubated for 30 min,and then challenged with ST.The effect of SHLI on β-hexosaminidase(β-HEX) release was determined by fluorescence spectrophotometry.KU812 cells were stimulated with phorbol-12-myristate 13-acetate(PMA) plus calcium inophore A23187.The effect of SHLI on pro-inflammatory cytokine(TNF-α,IL-6 and PGE2) production in the PMA/A23187 stimulated KU812 cells was measured by ELISA.The mouse paw swelling was induced by anti-ST-serum and ST.The effect of SHLI(0.5%,1.0% and 2.0%)on the volume and the volume change of the paw was measured at different time points(1 h and 24 h)after ST challenge.RESULTS Compared with model group,SHLI(0.5%,1.0% and 2.0%) inhibited the degranulation of RBL-2 H3 cells and the fluorescence value decreased by 16%,31% and 51% respectively(P<0.01).SHLI reduced PMA/A23187-induced inflammatory cytokine production in KU812 cells(P<0.01),with the inhibitory rate of 35%,55% and 69% for TNF-α,29%,51% and 74% for IL-6,and31%,40% and 55% for PGE2,respectively.The in vivo results showed that SHLI(2.5 and 5.0 mL·kg^-1)decreased the volume change of the paw in both immediate-phase(1 h) and late-phase(24 h) in mice,by 69% and 83% 1 h after ST challenge(P<0.01),and by 70%(P<0.05) and 100%(P<0.01) 24 h after ST challenge.CONCLUSION SHLI can inhibit the inflammatory response in the ST-induced typeⅠhypersensitivity.

关 键 词：双黄连注射液 Ⅰ型超敏反应 炎症反应 虾原肌球蛋白 

分 类 号：R285.5[医药卫生—中药学] R967[医药卫生—中医学]