叶黄素对人前列腺癌PC-3M细胞增殖抑制作用  

Inhibitory effect of lutein on proliferation of human prostate cancer PC-3M cells

作　　者：叶兴龙[1] 赵丽晶 金丁健 YE Xing-long;ZHAO Li-jing;JIN Ding-jian(Department of Urology, First Affiliated Hospital of Beihua University, Jilin, Jilin Province 132013, China)

机构地区：[1]北华大学第一临床附属医院泌尿外科,吉林吉林132013 [2]吉林医药学院基础医学院基础医学实验教学中心 [3]北华大学临床学院

出　　处：《中国公共卫生》2018年第12期1642-1645,共4页Chinese Journal of Public Health

基　　金：吉林省教育厅"十三五"科学技术项目(JJKH20170048KJ);吉林省教育厅"十二五"科学技术研究项目(2015396)

摘　　要：目的探讨叶黄素对体外培养人前列腺癌PC-3M细胞增殖的抑制作用及机制。方法选择人前列腺癌PC-3M细胞为研究对象,随机分为5组:0(对照组)、5、10、20、40μmol/L叶黄素组,采用噻唑蓝法检测PC-3M细胞增殖抑制率,采用流式细胞术检测PC-3M细胞周期,采用荧光显色法观察细胞形态,紫外分光光度法检测细胞上清液中caspase-3的含量。结果与对照组比较,各剂量叶黄素组PC-3M细胞增殖抑制率明显升高,具有剂量和时间依赖性(P <0.01);与对照组比较,叶黄素10、20、40μmol/L剂量组PC-3M细胞G1期细胞比例明显增加,G2M期细胞比例明显减少(P <0.01);与对照组比较,叶黄素各剂量组PC-3M细胞形状发生明显变化,细胞变圆,核碎裂且核染色质凝聚,凋亡小体形成,且随剂量增加凋亡数显著升高;与对照组比较,各剂量叶黄素组PC-3M细胞上清液中caspase-3含量明显升高,呈剂量效应关系(P <0.05)。结论叶黄素可抑制体外培养的人前列腺癌PC-3M细胞的增殖,诱导其凋亡,其机制可能与叶黄素增加PC-3M细胞caspase-3表达有关。Objective To investigate inhibitive effect of lutein on the proliferation of human prostate cancer PC-3M cells and the mechanism of the effect in vitro.Methods Human prostate cancer PC-3M cells were randomly divided into a control group and 4 lutein treatment groups at doses of 5, 10, 20, and 40 μmol/L. The inhibitive rate of PC-3M cells proliferation was measured with methyl thiazolyl tetrazolium(MTT)assay;the cell cycle of PC-3M cells was detected with flow cytometry;the cell morphology was observed with fluorescence development;and the content of caspase-3 in the cell culture supernatant was measured with ultraviolet spectrophotometry.Results Compared with that in the control group, the inhibitive rate of the PC-3M cells' proliferation was significantly increased in all lutein dose groups in a dose- and time-dependent manner(P<0.01). The proportion of the cells in G1 phase increased but that in G2M phase decreased significantly for the PC-3M cells treated with lutein at various doses in comparison with that of cells of control group(both P<0.01). Significantly changed morphologies were observed in lutein-treated PC-3M cells, including rounded cell shape, nucleus fragmentation, condensed nuclear chromatin, and formation of apoptotic bodies, in contrast to those of the control cells;the number of apoptotic cells increased significantly with the increment of lutein doses. The content of caspase-3 in culture supernatant of PC-3M cells treated with various doses of lutein was significantly higher than that of control cells and the increase of caspase-3 was positively correlated with the increment of lutein dose significantly(P<0.05).Conclusion Lutein can inhibit the proliferation and induce apoptosis of human prostate cancer PC-3M cells in vitro;the mechanism of the effect may be related to the increased expression of caspase-3 in the lutein-treated PC-3M cells.

关 键 词：前列腺癌 PC-3M细胞 叶黄素 细胞增殖 细胞凋亡 

分 类 号：R737.25[医药卫生—肿瘤]