基于磁弹性生物传感器检测癌胚抗原  

Detection of Carcinoembryonic Antigen Based on Magnetoelastic Biosensors

作　　者：郭锦玉 张益霞 郭星 李艳萍[1,2] Guo Jinyua;Zhang Yixia;Guo Xing;Li Yanping(Micro-Nano System Research Center,College of Information and Computer Science,Taiyuan niversity of Technolgy ,Jinzhong030600,China;Key Laboratory of Advanced Transducers and Intelligent Control Systerm of the Ministy of Education,Taiyuan niversity of Technolgy ,Jinzhong030600,China;Institute of Applied Mechanics and Biomedical Engineering,College of Mechanics,Taiyuan University of Technolgy ,Jinzhong030600,China)

机构地区：[1]太原理工大学信息与计算机学院微纳系统研究中心,山西晋中030600 [2]太原理工大学新型传感器与智能控制系统教育部(山西省)重点实验室,山西晋中030600 [3]太原理工大学力学学院应用力学与生物医学工程研究所,山西晋中030600

出　　处：《微纳电子技术》2019年第1期51-56,共6页Micronanoelectronic Technology

基　　金：国家自然科学基金资助项目(61471255,51622507,61474079,81602506)

摘　　要：基于磁致伸缩材料将巯基十一烷酸自组装于磁弹性芯片,经N-羟基丁二酰亚胺(NHS)和1-乙基-(3-二甲基氨基丙基)碳二亚胺盐酸盐(EDC)活化后固载癌胚抗原抗体(AntiCEA),设计构建了一种新型且低成本的磁弹性生物传感器,基于抗原-抗体特异性识别以及传感器质量增加其共振频率降低的机理,实现对癌胚抗原(CEA)的检测。采用荧光显微镜对异硫氰酸荧光素标记的癌胚抗原抗体(FITC/Anti-CEA)功能化浓度进行了优化,采用原子力显微镜(AFM)和扫描电子显微镜(SEM)对裸金层、自组装膜(SAM)、Anti-CEA负载以及CEA检测进行了表征。实验结果表明,Anti-CEA最佳修饰质量浓度为50μg/mL,CEA线性响应范围为200~1000 ng/mL,检测下限为200 ng/mL。该磁弹性生物传感器有望实现高灵敏快速癌症标志物CEA的检测。The thiol undecanoic acid was self-assembled on the magneticelastic chip based on magnetostrictive materials,and activated with the EDC and NHS to immobilize the Anti-CEA,and then a novel and low-cost magnetoelastic biosensor was designed.Based on the antigen-antibody specific recognition and the mechanism of the resonance frequency reduction with the increase of the sensor quality,the carcinoembryonic antigen(CEA)was detected.The functional concentration of the FITC/Anti-CEA was optimized by the fluorescence microscope.The atomic force microscope(AFM)and scanning electron microscope(SEM)were used to characterize the bare gold layer,self-assembled film(SAM),Anti-CEA immobilization and CEA detection.The experimental results show that the optimal modification mass concentration of the Anti-CEA is50μg/mL,the linear response range of the CEA is 200-1 000 ng/mL,and the low detection limit is 200 ng/mL.The magnetoelastic biosensor will be applied in highly sensitive and rapid CEA detection.

关 键 词：磁致伸缩材料 自组装 癌胚抗原(CEA) 共振频率 磁弹性生物传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]