用于线虫药物筛选的微流控梯度芯片和自动化平台  

Microfluidic Gradient Chip and Automation Platform for Drug Screening of C. elegans

作　　者：张维军 赵世坤[1] 于洋[1] 何希文 奚晶 栾洋[2] 李遥 陈翔[1] Zhang Weijun;Zhao Shikun;Yu Yang;He Xiwen;Xi Jing;Luan Yang;Li Yao;Chen Xiang(National Key Laboratory of Science and Technology on Micro /Nano Fabrication,Department of Micro/Nano Electronics,Shanghai Jiao Tong University ,Shanghai200240,China;School of Public Health,School of Medicine,Shanghai Jiao Tong University,Shanghai 200336,China;East China University of Political Science and Law,Shanghai 201620,China)

机构地区：[1]上海交通大学微纳电子学系微米/纳米加工技术国家级重点实验室,上海200240 [2]上海交通大学医学院公共卫生学院,上海200336 [3]华东政法大学,上海201620

出　　处：《微纳电子技术》2019年第1期78-84,共7页Micronanoelectronic Technology

基　　金：上海市科委基础重大研发计划资助项目(17JC1401001)

摘　　要：作为一种在微米尺度操作流体的技术,微流控芯片具有反应体系小、通量高、自动化且操作灵活等优势,被越来越多地应用于细胞和微米尺度生物的研究中。秀丽隐杆线虫作为一种重要的模式生物被广泛地用于神经生物学、衰老及发育和药物筛选等研究中。提出了一种用于研究线虫和环境毒素相互作用的微流控自动化平台,该平台集成了基于微振荡原理的快速梯度形成的微流控芯片、自动化控制系统及基于OpenCV的线虫长度及摆动频率估计的自动化图像分析软件。通过染料和荧光实验验证了基于振荡原理的快速梯度形成芯片,该芯片可以在7min内形成线性浓度梯度,并通过该芯片和平台验证了线性浓度梯度的双氧水对秀丽隐杆线虫活性的影响。As a technology for operating fluids on the micrometer scale,microfluidic chips have the advantages of small reaction system,high throughput,automation and flexible operation,and are increasingly used in the research of cells and microscale organisms.As an important model organism,the C.elegans are widely used in the research of neurobiology,aging,development and drug screening.The microfluidic automation platform for the study of the interaction between C.elegans and environmental toxins was presented.The platform integrated the fast gradient forming microfluidic chip based on the micro-oscillation principle,automatic control system and OpenCV-based software for automatic image analysis of C.elegans length and wobble frequency estimation.The fast gradient forming chip based on the oscillation principle was verified by dye and fluorescence experiments.The chip can form a linear concentration gradient within 7 min.The effect of the linear concentration gradient hydrogen peroxide on the activity of C.elegans was verified by the chip and platform.

关 键 词：微流控芯片 秀丽隐杆线虫 自动化平台 药物筛选 环境毒素 

分 类 号：O35[理学—流体力学]