天门冬水煎剂对氟染毒大鼠脑组织SIRT1和BDNF表达的影响  

Effects of asparagus decoction on expressions of SIRT1 and BDNF in brain tissues of rats exposed to fluoride

作　　者：王飞清 李艳菊 刘燕青[1] 杨华[1] 李丽[1] 李桂筑 刘洋[1] WANG Fei-qing;LI Yan-ju;LIU Yan-qing;YANG Hua;LI Li;LI Gui-zhu;LIU Yang(Department of Clinical Laboratory,The First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine,Guiyang,Guizhou 550001,China;Department of Hematology,The Affiliated Hospital of Guizhou Medical University,Guiyang,Guizhou 550004,China)

机构地区：[1]贵阳中医学院第一附属医院检验科,贵州贵阳550001 [2]贵州医科大学附属医院血液科,贵州贵阳550004

出　　处：《环境与职业医学》2018年第12期1129-1133,共5页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金项目(编号:31660326);贵州省科技厅项目(编号:黔科合LH字[2016]7511;[2017]7151号);贵州省教育厅青年科技人才成长项目(编号:黔教合KY[2016]190);贵州省中医药管理局项目(编号:QZYY-2018-018);贵阳中医学院基金[2017]12;13号

摘　　要：[目的]探讨天门冬水煎剂对氟染毒大鼠脑组织沉默信息调节因子2相关酶1(SIRT1)和脑源性神经营养因子(BDNF)表达的影响。[方法]将清洁级SD大鼠60只随机分为对照组(自由食用正常玉米饲料)、染氟组(食用50 mg/kg含氟饲料)、天门冬低剂量治疗组(2.5 g/kg天门冬+50 mg/kg含氟饲料)、天门冬中剂量治疗组(5 g/kg天门冬+50 mg/kg含氟饲料)、天门冬高剂量治疗组(10 g/kg天门冬+50 mg/kg含氟饲料),每组12只,雌雄各半。以自由食用含氟饲料方式进行染毒,并以灌胃方式给药天门冬水煎剂,每天1次,1 mL/次,连续3个月,采用实时定量荧光聚合酶链反应(qRT-PCR)和免疫印迹法检测脑组织SIRT1、BDNF的mRNA和蛋白表达水平。[结果]染氟组和不同剂量天门冬组大鼠脑组织SITR1、BDNF的mRNA和蛋白水平均低于对照组,且差异均有统计学意义(均P<0.05)。天门冬中剂量和高剂量组大鼠脑组织SITR1、BDNF的mRNA和蛋白表达水平均高于染氟组,差异有统计学意义(P<0.05)。各天门冬剂量组比较,随天门冬剂量增加,大鼠脑组织SITR1、BDNF的mRNA和蛋白表达水平逐渐升高(P<0.05)。光镜下观察染氟组大鼠脑组织星形细胞明显减少,细胞出现肿胀,核仁消失,呈现明显损伤的现象;各天门冬组大鼠脑组织星形细胞逐渐增加,核仁逐渐清晰,较染氟组明显改善。[结论]在本实验剂量下,天门冬表现出一定的脑保护作用,可能与天门冬上调氟染毒大鼠脑组织SITR1、BDNF的mRNA和蛋白水平有关。[Objective] To investigate the effects of asparagus decoction on the expressions of silent mating type information regulation 2 homolog 1 (SIRT1) and brain-derived neurotrophic factor (BDNF) in brain tissues of rats exposed to fluoride.[Methods] Sixty clean SD rats were randomly divided into control group (corn feed), fluoride group (fluoride 50mg/kg), asparagus low-dose treatment group (asparagus 2.5 g/kg + fluoride 50 mg/kg), asparagus moderate-dose treatment group (asparagus 5 g/kg + fluoride 50 mg/kg), and asparagus high-dose treatment group (asparagus 10 g/kg + fluoride 50 mg/kg), with 12 rats in each group, half male and half female. The rats were exposed to fluoride by free feeding and administered by gavage with asparagus decoction, once a day, 1 mL/time, for three continuously months. Brain SIRT1 and BDNF mRNA and protein levels were detected by real-time quantitative PCR and Western blot.[Results] The levels of SITR1 and BDNF mRNA and protein in brains of rats exposed to fluoride and different doses of asparagus were lower than those in the control group (Ps < 0.05). The levels of SITR1 and BDNF mRNA and protein in brains of the moderateand high-dose asparagus treatment groups were lower than those in the fluoride group (Ps < 0.05). The levels of SITR1 and BDNF mRNA and protein in rat brains increased gradually with increasing asparagus dose (P < 0.05). Under optical microscopy, reduced astrocytes in the brain tissues of rats exposed to fluoride were observed, as well as swollen cells and disappearing nucleolus, showing obvious damage; increasing astrocytes in the brain tissues of rats treated with asparagus were observed, as well as clear nucleolus compared with the fluoride group.[Conclusion] Asparagus at designed doses has a protective effect on brain tissues post floride exposure, and the mechanism may be related to the up-regulation of mRNA and protein levels of SITR1 and BDNF in brain tissues of rats exposed to fluoride.

关 键 词：氟染毒 天门冬 脑 沉默信息调节因子2相关酶1 脑源性神经营养因子 

分 类 号：R114[医药卫生—卫生毒理学]