血清HCV-RNA载量及抗-HCV浓度检测在丙型肝炎临床诊断中的应用分析  

Application of serum HCV-RNA load and anti-HCV concentration detection in clinical diagnosis of hepatitis C

作　　者：白娜[1] BAI Na(Department of Ciinical Laboratory,Pingdingshan First People's Hospital,Pingdingshan,467000,China)

机构地区：[1]平顶山市第一人民医院检验科,河南平顶山467000

出　　处：《临床血液学杂志（输血与检验）》2018年第6期922-924,共3页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

摘　　要：目的:探讨血清HCV-RNA载量及抗-HCV浓度检测在丙型肝炎临床诊断中的应用。方法:选取2017-04—2018-04治疗的丙型肝炎患者100例纳入试验组,选取同期30例健康人群纳入对照组。采用荧光定量PCR法对其进行HCV-RNA载量检测,ELISA法检测抗-HCV浓度、全自动生化分析仪检测血清中AST、ALT、TBIL的浓度,并进行统计学分析。结果:100例标本中抗-HCV阳性者89例,阳性率为89%;随着HCV-RNA载量的不断增加,抗-HCV阳性率不断升高,差异有统计学意义(P<0.05);试验组的抗-HCV s/co值高于对照组,差异有统计学意义(P<0.05);试验组的血清AST、ALT、TBIL值均明显高于对照组,差异有统计学意义(P<0.05)。结论:HCV-RNA载量联合抗-HCV及肝功能指标检测,不仅可有效对患者的感染、病情严重程度做出准确判断,还可以对临床治疗进行监测。Objective:To investigate the application of serum HCV-RNA load and anti-HCV concentration detection in the clinical diagnosis of hepatitis C.Method:A total of 100 patients with hepatitis C treated with 2017-04-2018-04 were enrolled in the experimental group.Thirty healthy people in the same period were selected as the control group.The HCV-RNA load was detected by real-time PCR,the anti-HCV concentration was detected by ELISA,and the concentrations of AST,ALT and TBIL in serum were detected by automatic biochemical analyzer,and statistical analysis was performed.Result:Of the 100 specimens,89 were positive for anti-HCV,and the positive rate was 89%.With the increase of HCV-RNA load,the positive rate of anti-HCV increased continuously,the difference was statistically significant(P<0.05).The anti-HCV s/co value of the experimental group was higher than that of the control group,and the difference was statistically significant(P<0.05).The serum AST,ALT and TBIL values of the experimental group were significantly higher than the control group,and the difference was statistically significant(P<0.05).Conclusion:HCV-RNA load combined with anti-HCV and liver function indicators can not only effectively determine the patient’s infection and severity,but also monitor clinical treatment.

关 键 词：丙型肝炎 HCV-RNA载量 抗-HCV浓度 检测 

分 类 号：R512.6[医药卫生—内科学]