人源抗M试剂血清的制备和应用  

Preparation and application of human anti-M antibody reagent serum

作　　者：张璐[1] 伍钢[1] 杜海林[1] ZHANG Lu;WU Gang;DU Hailin(Nanjing Red Cross Blood Center,Nangjing,210003,China)

机构地区：[1]南京红十字血液中心研究室,南京210003

出　　处：《临床血液学杂志（输血与检验）》2018年第6期933-935,共3页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

基　　金：南京市医学科技发展一般性课题(No:ykk15186)

摘　　要：目的:利用南京红十字血液中心废弃抗M抗体阳性血浆制备人源抗M试剂血清,达到报废血液再利用的目的。方法:将抗M抗体阳性的血浆通过中和稀释,冻融浓缩,海藻糖-PEG浓缩等一系列方法,制备成人源化的抗M试剂血清。结果:制备的血清抗M效价可提高4倍,与市售试剂血清相比凝集强度有提高,1～2个月后制备的抗体效价基本不变。结论:利用废弃抗M抗体阳性血浆制备的人源抗M试剂血清亲和力在一定程度上高于市售血清,可适当用于日常标本检测,达到废血再利用的目的。Objective:To prepare human anti-M antibody reagent serum using the discarded anti M antibody positive plasma in the center,so as to achieve the purpose of discarding blood for reuse.Method:Human anti-M antibody reagent serum was prepared by neutralization dilution,freeze thawing concentration,trehalose-PEG concentration and so on.Result:The serum titer of anti-M could be increased by 4 times,and the agglutination intensity was higher than that of commercially available reagent serum.The titer of antibody prepared was basically un- changed after 1-2 months.Conclusion:The serum affinity of human anti-M reagent prepared by the anti M antibody positive plasma was higher to some extent than that of the marketed sera,and it co be used to test the daily specimens properly to achieve the purpose of reusing the waste blood.

关 键 词：人源抗M试剂血清 冻融浓缩 海藻糖-PEG 

分 类 号：R457.1[医药卫生—治疗学]