罕见B(A)02/O01致疑难血型鉴定  

Identification of rare B(A)02/O01 causing difficult blood type

作　　者：许进明[1] 余悦娇[1] 史丽莉[2] 周小玉[1] XU Jinming;YU Yuejiao;SHI Lili;ZHOU Xiaoyu(Department of Blood Transfusion,First Affiliated Hospital of Nanjing Medical University,Nanjing,210000,China;Jiangsu Blood Center)

机构地区：[1]南京医科大学第一附属医院输血科 [2]江苏省血液中心

出　　处：《临床血液学杂志（输血与检验）》2018年第6期960-962,共3页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

摘　　要：目的:准确掌握B(A)血型血清学反应格局,熟知其形成的分子机制,正确进行血型鉴定,保证临床输血的安全。方法:采用传统血型血清学方法对样本进行ABO血型鉴定,同时运用PCR-SSP方法对标本ABO基因进行分析。结果:血型血清学正定型为A弱B,反定型为B型,Rh分型为DEecc,直接抗人球蛋白试验及血型不规则抗体筛查均为阴性,基因分型结果为B(A)02/O01型。结论:对于ABO正反定型不符的样本,基因分型技术的应用有助于解决疑难血型的鉴定,但熟练掌握并正确运用血型血清学技术也是必不可少的。Objective:To accurately master the reaction pattern of B (A)blood group serology,and know the molecular mechanism that it forms very well,so as to make correct determination of blood group and guarantee the safety of clinical blood transfusion.Method:The traditional blood group serological method was used to identify ABO blood group of samples,and PCR-SSP method was used to analyze ABO gene of the specimen.Result:The di-rect typing of blood group serology was A weak B,reverse typing B type and Rh type DEecc.The direct anti human globulin test and blood group irregular antibody screening were all negative.The result of genotyping was B (A)02/O01type.Conclusion:The application of genotyping may be helpful to the identification of difficult blood groups for samples not complying with ABO direct and reserve stereotypes,but it should be essential to master and use blood group serological techniques skillfully.

关 键 词：B(A) 疑难血型 基因分型 

分 类 号：Q343.1[生物学—遗传学]