脐带华氏胶来源间充质干细胞及其分泌的可溶性因子IDO的免疫抑制作用  

Immunosuppressive Properties of Wharton's Jelly Derived Mesenchymal Stromal Cells and Secreted Soluble Factors IDO to T Lymphocytes

作　　者：何海萍 HE Haiping(Medical School,Kunming University of Science and Technology,Kunming 650500,China;Department of Hematology,First people's Hospital of Yunnan Province,Kunming 650032,China)

机构地区：[1]昆明理工大学医学院,云南昆明650500 [2]云南省第一人民医院血液科,云南昆明650032

出　　处：《昆明理工大学学报（自然科学版）》2018年第6期96-102,共7页Journal of Kunming University of Science and Technology(Natural Science)

基　　金：国家自然科学基金地区项目(81760028);昆明理工大学校人才培养项目(kksy201560048);昆明医科大学应用基础研究联合专项(2018FE001-129)

摘　　要：探讨人脐带华氏胶来源的间充质干细胞(WJ-MSCs)及其分泌的可溶性因子IDO发挥的免疫调节作用.本研究利用人脐带华氏胶分离培养间充质干细胞,从健康人脐带血、外周血分离获取单个核细胞,通过流式分析来检测细胞表型,通过与T淋巴细胞直接接触培养及Transwell间接培养观察混合淋巴细胞反应(MLR)中WJ-MSCs的免疫调节作用,并利用IDO抑制剂1甲基色氨酸(1MT)来检测其所发挥的干预作用.研究表明:WJ-MSCs阳性表达CD73、CD90、CD105、HLA-ABC;阴性表达CD45、CD34、HLA-DR.在混合淋巴细胞反应中,反应性T淋巴细胞的增殖可以被来自不同供者或是来自第三方的间充质干细胞有效的抑制. WJ-MSCs的免疫抑制作用是通过与淋巴细胞直接接触及释放的可溶性细胞因子共同实现的,与Transwell间接培养相比较,与T淋巴细胞直接接触培养的WJ-MSCs表达了更强的免疫抑制效果,同时WJMSCs的抑制作用可被IDO抑制剂部分逆转,这表明IDO是WJ-MSCs分泌的可溶性免疫抑制因子之一.This paper investigates the immunomodulatory effect of human umbilical cord warton’s jelly derived mesenchymal stem cells(WJ-MSCs) and its soluble factor IDO. WJ-MSCs and mononuclear cells were isolated from normal human cord blood and peripheral blood. Flow cytometry was used to detect cell phenotype and to observe the immunomodulatory effect to WJ-MSC on mixed lymphocyte reaction(MLR) by direct culture and Transwell indirect culture. IDO inhibitor 1 methyltryptophan(1 MT) was used to detect its role in immunosuppression. Our research shows that,WJ-MSCs were positive in CD73,CD90,CD105 and HLA-ABC,but negative in CD45,CD34 and HLA-DR. In MLR,the proliferation of autologous or allogeneic responder cells can be effectively inhibited by WJ-MSCs. Moreover,3 rd party-derived WJ-MSCs also strongly suppressed allogeneic MLR. WJ-MSCs expressed immunosuppressive effect by contact with cells and soluble cytokines.Compare with Transwell indirect culture method,WJ-MSCs direct contact with T lymphocyte expressed stronger immunosuppressive effect. The inhibitory effect of WJ-MSCs can be partially reversed by IDO inhibitor. IDO is an effective Immunosuppressive factor that secreted from WJ-MSCs.

关 键 词：脐带间充质干细胞 混合淋巴细胞反应 T淋巴细胞 免疫抑制作用 

分 类 号：R392.4[医药卫生—免疫学]