HBsAg与anti-HBs共阳性慢性乙肝患者病毒S蛋白序列分析  

Sequence of S protein in chronic hepatitis B patients with coexisting HBsAg and anti-HBs

作　　者：申春梅[1] 唐思叶 王妹芳[1] 贺乐奇[1] SHEN Chun-mei;TANG Si-ye;WANG Mei-fang;HE Le-qi(Shanghai Fifth People's Hospital Affiliated to Fudan University,Shanghai 200240,China)

机构地区：[1]复旦大学附属上海市第五人民医院,上海200240

出　　处：《实用预防医学》2018年第12期1458-1461,共4页Practical Preventive Medicine

基　　金：上海市闵行区自然科学基金资助项目(2014MHZ040)

摘　　要：目的分析慢性乙型肝炎(chronic hepatitis B,CHB)患者乙型肝炎病毒S蛋白序列突变与HBs Ag、anti-HBs共阳性的相关性。方法随机选择33例HBs Ag、anti-HBs共阳性的CHB患者作为试验组,随机选择33例HBs Ag阳性而anti-HBs阴性的CHB患者作为对照组,对乙型肝炎病毒S区基因进行扩增测序并进行序列分析。结果两组感染的乙型肝炎病毒均为B或C基因型,未见其它基因型;两组在年龄、性别、HBV基因型、血清HBV DNA水平上差异无统计学意义(均P>0. 05);试验组S蛋白在N-端(2. 61%vs. 1. 21%)、主要亲水结构区域(1. 69%vs. 0. 48%)(major hydrophilic region,MHR)、特别是"α"决定簇(3. 23%vs. 0. 63%)的氨基酸突变率均显著高于对照组(均P<0. 01),但在C-端(1. 75%vs. 1. 22%)氨基酸突变率差异无统计学意义(P>0. 05);试验组突变率最高的点是S蛋白的第126位氨基酸;两组氨基酸插入和缺失突变差异无统计学意义(P>0. 05)。结论 CHB患者中S蛋白序列氨基酸的突变特别是"α"决定簇的突变可能与HBs Ag、anti-HBs共阳性相关。Objective To analyze the correlation between the mutation of S protein sequence of hepatitis B virus (HBV) and the coexistence of HBsAg and anti-HBs in patients with chronic hepatitis B (CHB). Methods Thirty-three CHB patients with coexisting HBsAg and anti-HBs were randomly selected as the experimental group, and 33 CHB patients with HBsAg positive but anti-HBs negative as the control group. The S gene of HBV was amplified and sequenced. Results HBV in the two groups was B or C genotype, without other genotypes. No statistically significant differences were observed in age, gender, HBV genotype and serum HBV DNA level between the two groups (all P>0.05). The amino acid mutation rates in the N-terminal (2.61% vs. 1.21%), major hydrophilic region (1.69% vs. 0.48%), especially "α" determinant (3.23% vs. 0.63%) of S protein were significantly higher in the experimental group than in the control group (all P<0.01), but no statistically significant difference was found in the amino acid mutation rate in the C-terminal of S protein (1.75% vs. 1.22%, P>0.05). The most frequent amino acid mutation in the experimental group was located at position 126 of S protein. There were no statistically significant differences in the deletion or insertion mutations between the two groups (P>0.05). Conclusions The amino acid mutations, especially those in the "α" determinant, in the patients with CHB may be associated with the coexistence of HBsAg and anti-HBs.

关 键 词：乙型肝炎表面抗原 乙型肝炎表面抗体 共阳性 S基因 突变 

分 类 号：R373.21[医药卫生—病原生物学]