结核分枝杆菌EF4蛋白在应激条件下的功能研究  

Function of Mycobacterium tuberculosis EF4 in response to stress

作　　者：顾晔星 陈金中[1] 沈琦[1] Gu Yexing;Chen Jinzhong;Shen Qi(State Key Laboratory of Genetic Engineering,Institute of Genetics,School of Life Sciences,Fudan University, Shanghai 200433,China)

机构地区：[1]复旦大学生命科学学院遗传工程国家重点实验室,上海200433

出　　处：《中华微生物学和免疫学杂志》2018年第12期886-890,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家重点研发计划专项(2016YFA0500600).

摘　　要：目的探讨结核分枝杆菌EF4蛋白在应激条件下的功能表现。方法将编码结核分枝杆菌EF4蛋白的pSMT-EF4质粒转入大肠杆菌BL21,使其表达结核分枝杆菌EF4蛋白,观察在不同应激条件下的生长情况,绘制其生长曲线。结果分别在含氨苄青霉素、G418和链霉素的培养基中,表达结核分枝杆菌EF4蛋白的大肠杆菌BL21的生长情况好于未表达结核分枝杆菌EF4蛋白的对照组,分别在6h、9h、2h后表现出显著性差异;在含H2O2以及pH3、20℃和45℃的培养基中,两者的生长情况无显著性差异。结论带有过表达结核分枝杆菌EF4蛋白突变的大肠杆菌BL21对抗生素类药物产生了一定的耐药性;结核分枝杆菌EF4蛋白没有直接参与氧化、酸性和温度的应激响应。Objective To investigate the function of Mycobacterium tuberculosis EF4 protein in response to stress. Methods The plasmid pSMT-EF4 encoding Mycobacterium tuberculosis EF4 protein was transformed into Escherichia coli (E.coli) BL21 to make it express EF4 protein. The successfully transformed E. coli BL21 strains were cultured under different stress conditions and the growth curves were recorded to study the function of EF4 protein in response to stress. Results In three media containing ampicillin, G418 and streptomycin respectively, the growth of E. coli BL21 expressing Mycobacterium tuberculosis EF4 protein was better than that of untransformed E. coli BL21, and significant differences were observed after culturing for 6 h, 9 h and 2 h, respectively. No significant difference in bacterial growth was found between experimental and control groups cultured under the condition of H2O2-containing medium, pH3, 20℃ or 45℃. Conclusion E. coli BL21 overexpressing Mycobacterium tuberculosis EF4 protein showed certain resistance to antibiotics. Mycobacterium tuberculosis EF4 protein was not directly involved in responses to oxidative, acidic and temperature stresses.

关 键 词：EF4蛋白 结核分枝杆菌 应激响应 抗生素耐药性 

分 类 号：R378.911[医药卫生—病原生物学]