神经肽Y在兔颈部交感神经节与脊神经节间通路应用研究  

The study of neuropeptide Y in neural pathway between cervical spinal ganglia and cervical sympathetic ganglia in rabbit

作　　者：韩建龙[1] 邱思强[1] 朱新炜[1] 栾方海[1] 常刚[1] 程继祖 左金良[1] HAN Jian-long;QIU Si-qiang;ZHU Xin-wei;LUAN Fang-hai;CHANG Gang;CHENG Ji-zu;ZUO Jinliang(Jinan Clinical College of Taishan Medical University /Department of Orthopedics, The 4th Hospital of Jinan, Jinan 250031, China;Department of Orthopedics, Sangzi Hospital of Jinan, Jinan 250031, China)

机构地区：[1]泰山医学院济南临床学院/济南市第四人民医院,济南250031 [2]济南市桑梓医院,济南250000

出　　处：《中国临床解剖学杂志》2018年第6期621-625,631,共6页Chinese Journal of Clinical Anatomy

基　　金：国家自然基金项目(81472138)

摘　　要：目的通过电刺激兔颈脊神经节,检测颈交感神经节内的神经肽Y(neuropeptideY,NPY)含量,研究颈交感神经节与颈脊神经节间的通路联系,探讨颈性眩晕发病机制中的神经解剖学基础。方法96只新西兰兔随机分为C2、C3、C4、C5、C6、C7组及相应对照组(每组8只),麻醉后显露相应的脊神经节,给予波宽0.5ms、频率30Hz、5V电刺激,5秒/次,每1min重复1次,共5次,30min后,灌注固定,切取双侧颈上、下交感神经节,切片,免疫反应,染色,观察。结果电刺激C2、C3脊神经节后,同侧颈上交感神经节内NPY含量升高(P<0.05);电刺激C4、C5脊神经节后,同侧颈上交感神经节和颈下交感神经节内NPY含量均有明显变化(P<0.05);电刺激C6、C7脊神经节后,同侧颈下交感神经节内NPY含量升高(P<0.05)。结论颈脊神经节和颈交感神经节间存在神经电生理上的通路联系,且具有节段性、同侧性特点,为颈性眩晕的发病机制及临床分型提供了实验依据和理论基础。Objective To study the neural pathway between cervical spinal ganglia and cervical sympathetic ganglia,and to explore the neural factors in the pathogenesis of cervical vertigo by detecting the neuropeptide Y(NPY)in cervical sympathetic ganglia after the cervical spinal ganglia electro-stimulated.Methods 96 New Zealand rabbits were divided into C2,C3,C4,C5,C6,C7and control groups randomly(n=8in each group).The electro-stimulations(width=0.5 ms,30 Hz,5 V,time=5 s,1 time/1 min,and 5 times total)were given to specific spinal ganglia which were revealed.After 30 min,the rabbits were killed through perfusion and the superior cervical sympathetic ganglia(SCG)and inferior cervical sympathetic ganglia(ICG)of both sides were cut.Then the section were made after immunologic reaction and observed under the microscope.ResultsThe content of NPY in the same side sympathetic ganglia was higher than the other side sympathetic ganglia and the control group sympathetic ganglia after the electro-stimulation was given to the cervical spinal ganglia,and the difference was statistic significance(P<0.05).When the electro-stimulation was given to the C2,C3spinal ganglia,the change of the NPY content of sympathetic ganglia were found in SCG mainly;when the electro-stimulation were given to the C4,C5spinal ganglia,the change of the NPY content of sympathetic ganglia were found in SCG and ICG;when the electro-stimulation were given to the C6,C7spinal ganglia,the change of the NPY content of sympathetic ganglia were found in ICG mainly.ConclusionThere is some kind of neural pathway between the cervical spinal ganglia and cervical sympathetic ganglia,and the characteristic of segment and homonymy in this kind of connection is also found,which provides a neuroanatomical basis for the hypothesis of reflex arc in cervical vertigo.

关 键 词：颈脊神经节 颈交感神经节 神经肽Y(NPY) 颈性眩晕 神经反射弧 

分 类 号：R338[医药卫生—人体生理学]