金针菇外切-β-1,3-葡聚糖酶家族基因鉴定及在不同发育时期的差异表达  

Identification of exo-β-1,3-glucanase family genes and differential expression of the genes at different developmental stages in Flammulina filiformis

作　　者：陶永新[1,2] 段静怡 仝宗军 严俊杰[2] 宋寒冰 谢宝贵[2] TAO Yong-Xin;DUAN Jing-Yi;TONG Zong-Jun;YAN Jun-Jie;SONG Han-Bing;XIE Bao-Gui(College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002,China)

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]福建农林大学菌物研究中心,福建福州350002

出　　处：《菌物学报》2018年第12期1661-1670,共10页Mycosystema

基　　金：福建农林大学园艺学院青年学术骨干培养基金(61201400706);福建农林大学科技创新专项基金(CXZX2017184);国家重点基础研究发展计划项目"食用菌产量和品质形成的分子机理及调控"(2014CB138302);木生型食用菌品种选育与产业化工程项目(fjzycxny2017010);福建省食用菌产业体系站长(K83139296)~~

摘　　要：金针菇具有很高的营养与保健价值,菌柄长短决定金针菇的产量与品质,而菌柄伸长的相关作用酶及分子机理尚不清楚。前期草菇中发现外切-β-1,3-葡聚糖酶基因(exg2)可能与菌柄伸长相关,但在金针菇中尚没有exg基因的相关报道。本研究首先在金针菇全基因组中鉴定到3个外切-β-1,3-葡聚糖酶家族基因(分别命名为:Ffexg1、Ffexg2、Ffexg3),并进行了克隆验证。进一步采用定量PCR对3个基因在金针菇不同发育时期及组织部位的差异性表达进行了分析。结果显示:Ffexg1只在菌柄中高表达,Ffexg2与Ffexg3在菌柄中表达量先上升后下降,在菌盖中呈逐渐上升趋势。3个Ffexg基因均在菌柄发生伸长的部位表达量较高,且在菇体水平放置后菌柄弯曲程度较大的部位表达量较高。结果显示金针菇exg家族3个基因存在时空差异性表达,在菌柄中伸长较快的时期及部位伴随着Ffexg基因的高表达。结合其功能预测,Ffexg家族基因可能作用于细胞壁成分β-1,3-葡聚糖链,从而在金针菇菌柄及菌盖发育中起作用。Flammulina filiformis is one of mushrooms with high nutritional and health value.Its yield and quality is determined by the length of the stipe.However,the related enzymes and molecular mechanism of the stipe elongation are still unclear.Previous investigation indicated that exo-β-1,3-glucanase gene(exg2)was associated with the elongation of stipe in Volvariella volvacea,however,there is no report about the exg gene in F.filiformis.In this study,three exo-β-1,3-glucanase family genes(named as Ffexg1,Ffexg2 and Ffexg3)were identified in the whole genome of F.filiformis,and the clone verification was Successful.Quantitative PCR was used to analyze the expression patterns of the three Ffexg genes at different developmental stages or tissues of F.filiformis fruiting bodies.The results showed that Ffexg1 was highly expressed in the stipe specifically.The transcription levels of Ffexg2 and Ffexg3 first increased and then decreased in the stipe,and gradually increased in the pileus.The three Ffexg genes were significantly expressed in elongating parts of the stipe,especially at notably bending part when horizontal placement of the fruiting bodies.It was indicated that there were temporal and spatial differences in the expression of the three Ffexg genes in F.filiformis,and the rapid elongation period of the stipe was accompanied by the higher transcription of the Ffexg genes.The Ffexg family gene may act on the β-1,3-glucan chain,a major component of cell wall,playing a role in the development of stipe and pileus in F.filiformis.

关 键 词：真菌形态建成 菌柄伸长 细胞壁作用酶 表达模式 

分 类 号：S646.15[农业科学—蔬菜学]