2株深海来源真菌次级代谢产物的研究  

Studies on the secondary metabolites of two deep-sea-derived fungi

作　　者：马新华[1] 郑卫敏 周芳[1] 孙凯慧 张海涛 钟添华[2] 骆祝华[2] 邵宗泽[2] 张永红[1] MA Xin-hua;ZHENG Wei-min;ZHOU Fang;SUN Kai-hui;ZHANG Hai-tao;ZHONG Tian-hua;LUO Zhu-hua;SHAO Zong-ze;ZHANG Yong-hong(School of Pharmacy,Fujian Medical University,Fuzhou 350122,China;Third Institute of Oceanography,State Oceanic Administration,Xiamen 361006,China)

机构地区：[1]福建医科大学药学院,福建福州350122 [2]国家海洋局第三海洋研究所,福建厦门361006

出　　处：《中国海洋药物》2018年第5期41-46,共6页Chinese Journal of Marine Drugs

基　　金：国家海洋局海洋生物遗传资源重点实验室开放课题(HY201604;HY201807);福建省科技创新平台项目(2018Y2001);福建医科大学启航项目(2016QH015)资助

摘　　要：目的对2株深海来源真菌Graphostroma sp. MCCC 3A00421和Aspergillus sp. MCCC 3A400414进行化学成分和生物活性研究。方法采用溶剂萃取、柱色谱层析及半制备HPLC等方法对2株深海真菌的发酵产物进行化学分离,通过理化性质及波谱学方法并参阅文献进行化合物结构鉴定,并初步评价其细胞毒活性。结果从菌株3A00421的发酵产物中分离得到了4个化合物,经鉴定其结构分别为3,5-dimethyl-8-methoxy-3,4-dihydroisocoumarin(1)、6-O-methylreticulol(2)、nectriapyrone(3)、demethyl nectriapyrone A(4);从菌株3A00414中分离得到了5个化合物,经鉴定其结构分别为(22E,24R)-麦角甾-5,7,22-三烯-3β-醇(5)、WIN 64821(6)、diorcinol(7)、9-十八碳烯酸(8)、9,12-十八碳二烯酸(9)。其中化合物6具有中等强度的细胞毒活性,其IC_(50)为7.14～15.78μmol/L。结论深海真菌是重要的活性物质来源和生物资源,本研究首次从深海来源Graphostroma属真菌的代谢产物中发现了2个异香豆素和2个苯并吡喃酮类化合物。Objective To study the chemical constituents and biological activities of two deep-sea-derived fungi,Graphostromasp.MCCC 3A00421 and Aspergillus sp.MCCC 3A00414.Methods The metabolites of two fungi were purified by solvent extraction,column chromatography and semi-preparative HPLC.The structures were established by physicochemical properties,spectral analysis,and comparison with the data of literatures.The proliferation inhibition activities were evaluated by MTT method.Results Four compounds were isolated from the EtOAc extract of the strain 3A00421 and identified as 3,5-dimethyl-8-methoxy-3,4-dihydroisocoumarin(1),6-O-methylreticulol(2),nectriapyrone(3),demethyl nectriapyrone A(4);and five compounds were isolated from 3 A00414:(22E,24R)-ergosta-5,7,22-trien-3β-ol(5),WIN 64821(6),diorcinol(7),9-octadecenoic acid(8),9,12-octadecadienoic acid(9).Compound 6 showed weak cytotoxic activities with IC50 ranging from 7.14 to 15.78μmol/L.Conclusion Deep-sea-derived fungi were important microbial resources of bioactive constituents.To date,this was the first discovery of two isocoumarins and two benzopyrones from the metabolites of Graphostromasp.from deep sea.

关 键 词：深海真菌 次级代谢产物 细胞毒活性 

分 类 号：R917[医药卫生—药物分析学]