转hFat-Ⅰ基因猪的繁殖性能及生长发育测定  

Determination of reproductive performance and growth development of hFat-Ⅰ transgenic pigs

作　　者：华再东[1] 肖伟 华文君[1] 毕延震[1] 肖红卫[1] 任红艳[1] 张立苹[1] 郑新民[1] HUA Zaidong;XIA0 Wei;HUA Wenjun;BI Yanzhen;XIAO Hongwei;REN Hongyan;ZHANG Liping;ZHENG Xinmin(Institute of Animal Husbandry and Veterinary Medicine/Hubei Province Key Laboratory of Animal Embryology and Molecular Breeding,Hubei Academy of Agricultural Sciences,Wuhan 430064,China;College of Animal Science,Yangtze University,Jingzhou 434025,China)

机构地区：[1]湖北省农业科学院畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室,武汉430064 [2]长江大学动物科学学院,湖北荆州434025

出　　处：《黑龙江畜牧兽医》2018年第24期40-42,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家转基因生物新品种培育重大专项(2016ZX08006002-006);动物胚胎工程及分子育种湖北省重点实验室开放课题(KLAEMB-2017-02);湖北省农业科技创新中心项目(2018-620-004-001)

摘　　要：为了探讨富含ω-3的转脂肪酸去饱和酶(Fat-Ⅰ)基因猪育种价值,试验选取转人源化Fat-Ⅰ(hFat-Ⅰ)基因阳性公猪和非转基因公猪各3头作为父本,通过人工授精方式分别与18头非转基因母猪配种测定其繁殖性能,并对转hFat-Ⅰ基因阳性猪和非转基因猪不同时期的生长发育情况进行测定和比较。结果表明:转hFat-Ⅰ基因猪和非转基因猪繁殖性能差异不显著(P>0. 05);转hFat-Ⅰ基因猪和非转基因猪0,25,32,64,110日龄的体重以及各生长阶段(0~25日龄、26~32日龄、33~64日龄)的日增重差异均不显著(P>0. 05)。说明外源hFat-Ⅰ基因对转基因猪的繁殖性能及生长发育无显著影响。In order to explore the breeding value of omega-3 fatty acid desaturase( Fat-Ⅰ) transgenic pigs,3 hFat-Ⅰ transgenic boars and 3 nontransgenic boars were selected as fathers in this experiment,artificial insemination for 18 non-transgenic sows was carried out and the reproductive performance was determined. The growth development of hFat-Ⅰ transgenic and non-transgenic pigs at different stages were measured and compared. The results showed that there was no significant difference( P>0. 05) on reproductive performance between hFat-Ⅰ transgenic pigs and non-transgenic pigs; the body weights at 0,25,32,64,110 days old and the daily gains at the different growth stages( 0 to 25,26 to 32,33 to 64 days old) had no significant differences( P>0. 05) between hFat-Ⅰ transgenic pigs and non-transgenic pigs. The results suggested that the exogenous hFat-Ⅰ gene had no significant effects on reproductive performance and growth development in transgenic pigs.

关 键 词：hFat-Ⅰ基因 转基因猪 生长发育 繁殖性能 脂肪酸去饱和酶(Fat-Ⅰ) 

分 类 号：S828.3[农业科学—畜牧学] Q785[农业科学—畜牧兽医]