杞红液预防臭氧吸入联合冰水浴诱导大鼠血管纤维化的实验研究  

Experimental study of Qihong decoction preventing vascular fibrosis in rats induced by ozone and icewater bath

作　　者：张睿 冯玄烨 王湘 戴宁 冯丽莉 赵欣[2] 蔡大勇[2] 王玥琦 ZHANG Rui;FENG Xuanye;WANG Xiang;DAI Ning;FENG Lili;ZHAO Xin;CAI Dayong;WANG Yueqi(School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China)

机构地区：[1]北京中医药大学中医学院,北京100029 [2]中国医学科学院药用植物研究所,北京100193

出　　处：《吉林中医药》2018年第12期1422-1425,共4页Jilin Journal of Chinese Medicine

基　　金：教育部重点研究基金资助项目(No.108019)

摘　　要：目的探究杞红液对臭氧吸入联合冰水浴诱导大鼠血管纤维化的影响。方法运用臭氧复合冰水浴造模法,制作寒凝血瘀衰老大鼠模型,检测血清NOS、MPO活力,ET-1含量;运用Masson染色和免疫组化来观察杞红液对动脉血管纤维沉积的影响。结果与对照组比较,模型组NOS活力降低(P <0.05),MPO活力升高(P <0.05),ET-1含量降低(P <0.05),血管纤维沉积增加(P <0.05),TGF-β1表达量升高(P <0.05)。与模型组比较,杞红液低、中、高剂量对上述指标、胶原纤维沉积、TGF-β1表达量有不同程度改善(P <0.05)。结论杞红液可能是通过调节大鼠NOS和ET-1的表达含量,调节机体应激反应,改善血管内皮功能及改善血管纤维化来实现对血管的保护作用。Objective To investigate the effect of Qihong decoction on arterial fibrosis by ozone inhalation and icewater bath in rats.Methods The rats with cold stagnation and blood stasis were made by ozone and ice.The level of serum nitric oxide synthase (NOS)activity,myeloperoxidase (MPO)endothelin-1 (ET-1)content,activity were detected.Morphometry of heart including of the volume ratio of cardiac muscle cell,deposition of arterial vascular cell collagen fibers of changes in rats were compared.The levels of the percentage of transforming growth factor beta 1 (TGF-β1)was detected in immunohistochemistry.Results Compared with control group,the activity of NOS decreased (P <0.05),the activity of MPO increased (P <0.05),the level of ET-1 content decreased (P <0.05), vascular fibrous deposition increased (P <0.05),TGF-β1 expression increased (P <0.05).Compared with the model group,the low,middle and high dose of Qihong decoction on the above indicators,collagen deposition,TGF-β1 expression in varying degrees of improvement (P <0.05).Conclusion Qihong decoction are expressed by adjusting the content of rat vascular NOS and ET-1,regulate the body's stress response,promote metabolism and improve endothelial function and improve vascular fibrosis to achieve the protective effect on vessels.

关 键 词：杞红液 臭氧 冰水浴 大鼠 血管 纤维化 转化生长因子Β1 

分 类 号：R285.5[医药卫生—中药学]