抗癌药紫杉醇与DNA相互作用的光谱研究  

Spectroscopic study on the interaction of paclitaxel with DNA

作　　者：邱烺[1] 梁静文 李康 QIU Lang;LIANG Jing-wen;LI Kang(School of Clinical Medicine,The First Affdiated Hospital of Guangdong Medical University,Guangzhou 510006,China;School of Pharmacy,Guangdong Pharmaceutical Univermty,Guangzhou 510006,China)

机构地区：[1]广东药科大学附属第一医院临床医学院,广东广州510006 [2]广东药科大学药学院,广东广州510006

出　　处：《分子科学学报》2018年第6期490-496,I0004,共8页Journal of Molecular Science

基　　金：国家自然科学基金资助项目(81573678);广东省自然科学基金资助项目(2015A030313584)

摘　　要：采用荧光光谱法和紫外-可见分光光谱法,在生理条件下(含0.1mol/LNaCl的Tris-HCl缓冲溶液,pH=7.40)研究了紫杉醇与生物大分子DNA的相互作用方式.结果表明DNA引起紫杉醇的紫外吸收光谱明显的红移和增色,并导致紫杉醇的荧光猝灭;测定不同温度下紫杉醇与DNA的荧光动态猝灭常数,结果显示K27℃=10266,K37℃=8755,K47℃=5788,即K27℃>K37℃>K47℃,说明动态猝灭常数随着温度升高而减小.同时考察了离子强度、磷酸根浓度及猝灭剂KI对紫杉醇与DNA相互作用的荧光强度的影响,三者对紫杉醇与DNA相互作用都无明显影响.表明DNA对紫杉醇的荧光猝灭类型是静态猝灭,紫杉醇与DNA的相互作用方式是嵌插作用.We investigated the spectral behavior of the interaction of paclitaxel with DNA.The fluorescence spectra and UV-vis spectroscopy were used to study the interaction between paclitaxel and biological macromolecule DNA under physiological conditions (Tris-HCl buffer solution containing 0.1 mol/L NaCl,pH=7.4).The results showed that the UV absorption spectra of paclitaxel were significantly red shift and hyperchromic effect,and fluorescence quenching of paclitaxel was caused by DNA.The fluorescence dynamic quenching constants of paclitaxel and DNA were measured at different temperatures with the K27℃,K37℃ and K47℃ value of 10 266,8 755 and 5 788,respectively,which indicated that K27℃>K37℃>K47℃.The effects of ion concentration,phosphate concentration and quencher KI on the fluorescence intensity of the in- teraction of paclitaxel and DNA were investigated at the same time.It was concluded that the fluorescence quenching type of DNA to paclitaxel was static quenching,and the interaction between paclitaxel and DNA was intercalation.

关 键 词：紫杉醇 DNA 紫外-可见分光光谱法 荧光光谱法 

分 类 号：O623[理学—有机化学]