蓝莓组培苗在新型生物反应器中的高效增殖技术  

Efficient Multiplication Technology of Vaccinium corymbosum Plantlets in New Type Bioreactors System

作　　者：宗树斌[1] 王永平[1] 顾立新[1] 陈少卿[1] 杨丽 陈晓波 ZONG Shubin;WANG Yongping;GU Lixin;CHEN Shaoqing;YANG Li;CHEN Xiaobo(Jiangsu Vocational College of Agriculture and Forestry,Jurong,Jiangsu 212400;Licheng Agriculture Service Center of Liyang,Liyang,Jiangsu 213300)

机构地区：[1]江苏农林职业技术学院,江苏句容212400 [2]溧阳市溧城镇农服中心,江苏溧阳213300

出　　处：《北方园艺》2018年第24期59-64,共6页Northern Horticulture

基　　金：江苏省林业三新工程资助项目(LYSX[2016]46);常州市农业三项更新工程资助项目

摘　　要：以蓝莓第3代组培苗为试材,采用对培养液体积、摇摆角度、接种密度3因素3水平L9(34)正交实验和对摇摆频率、浸没时间二因素完全随机试验方法,研究了蓝莓组培苗在新型摆动式间歇浸没生物反应器(S-TIBs)中的高效增殖技术,以期建立蓝莓组培苗在新型生物反应器中增殖培养的技术体系。结果表明:培养液体积每瓶250mL,摇摆角度45°,接种密度每瓶60株为最佳的培养参数,摇摆频率因素最佳为单次6h,浸没时间设定为60s。The third generation of blueberry plantlet was used as test material.The efficient multiplication technology of blueberry plantlets in S-TIBs was systematically studied through L9(3^4) orthogonal experiment with three factors and three levels (culture solution volume,swing angle and inoculation density) and completely randomized experiment with two factors (swing frequency and immersion time).The results showed that the optimal culture parameters were set as follows,the culture medium volume of 250 mL each bottle,the swing angle at 45°,and the inoculation density of 60 plantlets each bottle.The optimal swing frequency was 6 hours each time,and the immersion time was set as 60 seconds.

关 键 词：蓝莓 间歇浸没 组培苗 

分 类 号：S663.203.6[农业科学—果树学]