大别山地区杜鹃花资源遗传多样性的ISSR分析  

Genetic Diversity of Rhododendron simsii Populations Located on Dabie Mountains Based on ISSR Markers

作　　者：张蓓林 任瑾 田时雨 李志良[1] 干建平[1] 王书珍[1] ZHANG Beilin;REN Jin;TIAN Shiyu;LI Zhiliang;GAN Jianping;WANG Shuzhen(Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization/Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains/College of Life Science,Huanggang Normal University,Huanggang,Hubei 438000;College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan,Hubei 430070)

机构地区：[1]大别山特色资源开发湖北省协同创新中心,经济林木种质改良与资源综合利用湖北省重点研究室,黄冈师范学院生命科学学院,湖北黄冈438000 [2]华中农业大学园艺林学学院,湖北武汉430070

出　　处：《北方园艺》2018年第24期79-84,共6页Northern Horticulture

基　　金：国家自然科学基金资助项目(31500995);经济林木种质改良与资源综合利用湖北省重点研究室开发基金资助项目(2017BX06);黄冈师范学院培育基金资助项目(201617803).

摘　　要：以大别山地区狮子峰、五脑山和吴家山的野生杜鹃花居群为试材,采用14个ISSR引物进行遗传多样性分析,以期研究不同生境环境对杜鹃花遗传多样性的影响。结果表明:大别山地区野生杜鹃花具有较高的遗传多样性。共扩增出132个位点,多态性位点共130个,多态性比率为98.48%,其中多态性位点数量最多的是"吴家山"居群,共检测到124个多态性位点,比率高达93.94%;"五脑山"居群最少,检测到102个多态性位点,比率仅为77.27%。3个群体的遗传一致度为0.798 3～0.901 4,遗传距离变化范围为0.103 9～0.225 2。总遗传变异程度(Ht)为0.373 2,群体内的遗传变异程度(Hs)为0.307 8,居群间的遗传分化系数(Gst)为0.175 2,即总遗传变异中只有17.52%的变异存在于群体间,群体内的遗传变异为82.48%。该研究丰富了大别山杜鹃花资源遗传多样性研究案例,也为进一步开发和利用大别山地区野生杜鹃花资源打下基础。ISSR markers had been used to investigate the genetic diversity of Rhododendron simsii populations located on Dabie Mountains(Shizifeng,Wunaoshan,and Wujiashan).The results showed that high genetic diversity existed in natural R.simsii populations in this region.Totally,132 bands had been amplified,and polymorphic bands were 130,with the polymorphic rate of 98.48%.Among these bands,124 polymorphic loci were detected in ‘Wujiashan’population with the largest polymorphic rate of 93.94%.However,only 102 polymorphic loci were detected in ‘Wunaoshan’population(77.27%).Among these three populations,genetic identify ranged between 0.798 3 and 0.901 4,while genetic distance ranged from 0.103 9 to 0.225 2.The total genetic variation(Ht)was 0.373 2,the degree of genetic variation(Hs)within population was 0.307 8,and the coefficient of genetic differentiation(Gst)among populations was estimated to be 0.175 2.In particular,only 17.52% of the total genetic variation existed among populations,and the genetic variation within populations was 82.48%.This study will enrich the case study of genetic diversity of Rhododendronresources in Dabie Mountains,and lay good foundation for further development and utilization of wild Rhododendronresources.

关 键 词：杜鹃花 大别山 遗传多样性 ISSR 

分 类 号：S685.21[农业科学—观赏园艺]