肾动脉内、外膜射频消融对比格犬肾动脉CD36和SR-A表达的影响  

Effect of renal artery radiofrequency ablation in endarterium or adventitia on the expression of CD36 and SR-A

作　　者：朱利杰[1,2] 高传玉 王宪沛[1,2] 齐大屯 白民富[1,2] 简冬冬 杨宏辉[1,2] ZHU Lijie;GAO Chuanyu;WANG Xianpei;QI Datun;BAI Mingfu;JIAN Dongdong;YANG Honghui(Department of Cardiology,Henan Provincial People's Hospital;Fuwai Center China Cardiovascular Hospital,Zhengzhou, Henan 450000,China)

机构地区：[1]河南省人民医院心血管内科 [2]阜外华中心血管病医院,河南省郑州市450000

出　　处：《中国动脉硬化杂志》2018年第12期1227-1232,共6页Chinese Journal of Arteriosclerosis

基　　金：河南省科技攻关项目(172102310291);中国心血管健康联盟-心脏康复与代谢治疗基金(CCA-CRMT-1615)

摘　　要：目的研究肾动脉内、外膜射频消融对肾动脉清道夫受体CD36和SR-A表达水平的影响,分析肾动脉射频消融导致肾动脉粥样硬化的潜在风险。方法选取健康比格犬14只,雌雄各半,分为实验组(10只)和对照组(4只),实验组两侧肾动脉随机分为内膜组(10条动脉)和外膜组(10条动脉)。内膜组和外膜组均分为术后1个月组(5条动脉)和术后3个月组(5条动脉),对照组分为术后1个月组(4条动脉)和术后3个月组(4条动脉)。行肾动脉HE染色、免疫组织化学染色观察肾动脉的病理改变;通过Western blot检测肾动脉CD36和SR-A表达水平。结果(1)肾动脉造影结果:术后1个月内膜组出现1例肾动脉明显狭窄,其余均未见肾动脉狭窄。(2)肾动脉病理改变:肾动脉射频消融后,HE染色可见肾动脉灼烧部位中膜平滑肌排列紊乱,内膜局部增厚,结构破坏;免疫组织化学结果可见肾动脉CD36表达聚集。对照组血管壁未见明显改变。(3)肾动脉CD36和SR-A表达:①术后1个月,内膜组(n=5)和外膜组(n=5)CD36和SR-A表达量明显多于对照组(n=4)(P均<0.001);内膜组(n=5)较外膜组(n=5)CD36和SR-A表达量增加,差异有显著性(P=0.040和P=0.007)。②术后3个月,内膜组(n=5)和外膜组(n=5)CD36和SR-A表达量明显高于对照组(n=4)(P均<0.001);而内膜组(n=5)较外膜组(n=5)CD36和SR-A表达量增加,差异有统计学意义(P均<0.001)。结论肾动脉射频消融有导致肾动脉狭窄的可能,内膜消融可能更容易出现肾动脉狭窄;肾动脉内、外膜射频消融均有导致肾动脉粥样硬化的潜在风险。Aim To study the effect of renal artery radiofrequency ablation in endarterium or adventitia on renal artery atherosclerosis by detecting expression levels of renal artery macrophage scavenger receptor ( CD36 and SR-A) . Methods 14 healthy beagles ( 7 male,7 female) were randomly divided into experimental group ( n = 10) and control group ( n = 4) ,and two arteries in every beagle of the experimental group were randomly assigned to endarterium group( 10 artery) and adventitial group( 10 artery) . At 1 month and 3 months follow-up,renal artery angiography was performed to observe the renal artery morphological change; HE staining and immunohistochemical staining were used to observe the renal artery pathological change; Western blot was used to detect the expression levels of renal artery macrophage scavenger receptor ( CD36 and SR-A) . Results ( 1) At 1 month follow-up,1 case of obvious renal artery stenosis was found in endarterium group. ( 2) In the experimental group after renal denervation,intimal structure of renal artery was discontinued by HE staining,but no change was found in the control group; Immunohistochemistry showed the higher expression of CD36 in renal artery of experimental group. ( 3) The expression of scavenger receptors ( CD36 and SR-A) in renal artery: ①At 1 month follow-up,the expression levels of scavenger receptor CD36 and SR-A were significantly higher in endarterium group ( n = 5) and adventitia group ( n = 5) than that in the control group ( n = 4) ( P<0.001) ; And the expression levels of scavenger receptor CD36 and SR-A were significantly higher in endarterium group ( n = 5) than that in adventitia group ( P= 0.040 and P= 0.007) ; ②At 3 months follow-up,the expression levels of scavenger receptor CD36 and SR-A were significantly higher in endarterium group ( n = 5) and adventitia group ( n = 5) than that in control group ( n = 4) ( P< 0.001) ; And the expression levels of scavenger receptor CD36 and SR-A were significantly higher in endarterium group ( n = 5) than that in adventi

关 键 词：肾动脉射频消融 CD36 SR-A 肾动脉粥样硬化 

分 类 号：R5[医药卫生—内科学]