孕妇不规则抗体检测结果分析的临床意义研究  

Study on the clinical significance of irregular antibody detection in pregnant women

作　　者：李京[1] 沈云青[2] 徐群[1] LI Jing;SHEN Yun-Qing;XU Qun(Blood Group Reference Laboratory of Shandong Provincial Blood Center,Ji'nan,Shandong 250014,China)

机构地区：[1]山东省血液中心血型参比研究室,山东济南250014 [2]山东省血液中心供血科,山东济南250014

出　　处：《中国妇幼保健》2018年第24期5741-5743,共3页Maternal and Child Health Care of China

基　　金：山东省医药卫生科技发展计划项目(2011HZ086)

摘　　要：目的研究孕妇RhD血型系统不规则抗体检测结果分析的临床意义。方法选取2016年10月-2018年10月于该中心检测的1348例孕妇及同期非孕女性体检者1273例,采取微柱凝胶血型定型卡和抗人球蛋白卡进行不规则抗体检测,比较不规则抗体筛查阳性率。对其中抗体阳性者采用试管抗人球方法检测抗体效价、Ig类型及37℃反应,比较不同孕周及孕次的孕妇不规则抗体筛查阳性率。结果孕妇不规则抗体筛查阳性17例,阳性率1.26%,同期非孕女性体检者不规则抗体筛查阳性4例,阳性率0.31%,两组差异具有统计学意义(P<0.05);不规则抗体筛查阳性孕妇中,抗体特异性抗E6例,阳性率35.29%,抗体效价1∶8~1∶512,Ig类型:IgG15例、IgM1例及IgM-IgG1例,37℃反应性:1+~4+;不规则抗体筛查阳性孕妇中,≤28孕周孕妇阳性率0.53%,29~37孕周孕妇阳性率3.31%,38~40孕周孕妇阳性率0.00%,差异具有统计学意义(P<0.05);不规则抗体筛查阳性孕妇中,初产妇阳性率0.72%,经产妇阳性率2.13%,差异具有统计学意义(P<0.05)。结论孕妇不规则抗体阳性率高于非孕女性,产前进行不规则抗体检测能有效预防新生儿溶血。Objective To research the clinical significance of Rh D blood group system irregular antibody detection in pregnant women.Methods A total of 1 348 pregnant women and 1 273 non-pregnant women were selected from October 2016 to October 2018,microcolumn gel test and antihuman globulin test were used to detect irregular antibodies,the positive rates of irregular antibodies were compared. The women with positive irregular antibodies underwent tube antihuman globulin test to detect antibody titer,Ig types,and 37℃ reactivity. The positive rates of irregular antibodies were compared among the pregnant women of different gestational weeks and times of pregnancy.Results The positive rate of irregular antibodies was 1. 26%( 17 women),which was statistically significantly higher than that in non-pregnant women during the same period( P<0. 05). Among the pregnant women with positive irregular antibodies,the positive rate of specific anti-E antibody was 35. 29%( 6 women),antibody titer was 1 ∶ 8-1 ∶ 512,the numbers of pregnant women with positive Ig G,Ig M,and Ig M-Ig G were 15,1,and 1,respectively,37℃ reactivity was 1+-4+. Among the pregnant women with positive irregular antibodies,the positive rates of positive irregular antibodies in pregnant women ≤28,29-37,and 38-40 gestational weeks were 0. 53%,3. 31%,and0. 00%,respectively,there was statistically significant difference( P< 0. 05). Among the pregnant women with positive irregular antibodies,the positive rates of irregular antibodies in primiparous women and multiparous women were 0. 72% and 2. 13%,respectively,there was statistically significant difference( P<0. 05). Conclusion The positive rate of irregular antibodies in pregnant women is higher than that in non-pregnant women,prenatal detection of irregular antibodies can effectively prevent neonatal hemolysis.

关 键 词：RHD血型 不规则抗体 孕妇 

分 类 号：R714[医药卫生—妇产科学]