胶体金恶性疟原虫检测试剂盒在非洲加蓬的临床应用  

Clinical application of colloidal-gold detection reagent for Plasmodium falciparum in Gabon of Africa

作　　者：刘纯[1] Ndoumadiamba ALFRED Mounzie Gou GNONDA LIU Chun;Ndoumadiamba ALFRED;Mounzie Gou GNONDA(Tianjin First Central Hospital Blood Bank, Tianjin 300192, China;Laboratoire de l’Hospital de la Cooperation Sino-Gabonaise, de Libreville, Gabon 3843)

机构地区：[1]天津市第一中心医院输血科,天津300192 [2]加蓬利伯维尔中加合作医院检验科,加蓬利伯维尔市3843

出　　处：《中国寄生虫学与寄生虫病杂志》2018年第6期679-680,共2页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：2017年2月至2018年2月,采集就诊于加蓬利伯维尔中加医院的疑似疟疾患者外周血,应用胶体金恶性疟原虫检测试剂盒(简称胶体金法)进行检测,以显微镜镜检法作为金标准,评价胶体金法的敏感性和特异性。共采集120例(中国人50例,当地人70例)疑似疟疾患者血样,胶体金法检测阳性61例,阳性率50.8%(61/120)。镜检法检测疟原虫阳性56例(其中恶性疟52例、间日疟3例、卵形疟1例),阳性率为46.7%(56/120),两种方法检测结果的差异无统计学意义(P> 0.05)。胶体金法检测的敏感性和特异性均较高,分别是98.4%和100%。Peripheral blood was collected from suspected malaria patients in the China-Gabonas Hospital of Libreville in Gabon, from February 2017 to February 2018. The blood samples were examined by the Colloidal-Gold Detection Kit for Plasmodium falciparum antigen. Meanwhile, microscopy was performed as the gold standard to evaluate the sensitivity and specificity of the kit. A total of 120 suspected patients participated in this study, including 50 Chinese cases and 70 local residents. The colloidal-gold method revealed a positive rate for P. falciparum of 50.8% (61/120), while microscopy revealed a positive rate for malaria parasite of 46.7% (56/120), including 52 with P. falciparum, 3 with P. vivax, and 1 with P. ovale. There was no significant difference between the two methods (P > 0.05). The sensitivity and the specificity of the Colloidal Gold Detection Kit were 98.4% and 100%, respectively, indicating a good performance for P. falciparum.

关 键 词：疟疾 恶性疟原虫 胶体金快速诊断 镜检法 加蓬 

分 类 号：R531.3[医药卫生—内科学]