MACC1基因沉默对卵巢异位内膜间质细胞增殖与凋亡的影响  

The impact of MACC1 gene silencing on the proliferation and apoptosis of ovarian ectopic endometrial interstitial cells

作　　者：骆婕 吴敏敏 范荻 荆永萍 陶莹 LUO Jie;Wu Min—min;Fan Di;Jing Yongping;Tao Ying(Obstetrics and Gynecolog Department, the First Affiliated Hospital of Guangdong Pharmaceutical University,Guangzhou 510080,Guangdong,China)

机构地区：[1]广东药科大学附属第一医院妇产科,广东广州510080

出　　处：《广东医学》2018年第24期3600-3604,共5页Guangdong Medical Journal

基　　金：广东省医学科研基金立项项目(编号:A2018560)

摘　　要：目的探讨运用RNA干扰技术沉默MACC1基因表达对子宫内膜异位症卵巢异位内膜间质细胞增殖与凋亡的影响。方法采用实时荧光定量逆转录-聚合酶链反应(qRT-PCR)检测子宫内膜异位症卵巢异位病灶组织中MACC1基因的表达;体外培养人卵巢异位内膜间质细胞,运用RNA干扰技术转染构建MACC1-siRNA(转染组),设立阴性siRNA转染至细胞(阴性对照组),不经处理的细胞为空白对照组。分别在转染48 h后,运用qRT-PCR技术检测MACC1 mRNA,用Western blot法检测MACC1、HGF、C-Met蛋白的表达情况,MTT法检测转染后细胞的增殖情况、流式细胞仪测定细胞凋亡。结果 MACC1 mRNA在卵巢异位病灶组织中表达较卵巢功能性囊肿组织中明显升高,差异有统计学意义(P <0. 05)。转染组细胞MACC1mRNA及MACC1、HGF、C-Met蛋白的表达较阴性对照组及空白对照组均显著下降(P <0. 05)。转染组细胞增殖活力明显下降,而凋亡率增加(P <0. 05)。结论抑制MACC1基因表达可通过HGF/C-Met信号通路降低子宫内膜异位症卵巢异位内膜细胞的增殖活力,诱导细胞凋亡。Objective To observe the effect of silencing metastasis -associated in colon cancer -1 gene by RNA interference on the proliferation and apoptosis of ovarian ectopic endometrial interstitial cells in endometriosis.Methods The expression of MACC1 gene in ectopic endometrium of ovarian endometriosis was detected by real-time qRT -PCR. Ovarian ectopic endometrial interstitial cells were cultured in vitro.The siRNA interference technology was used to trans-fect MACC1 siRNA to the cells (the transfected group),while negative siRNA was transfected to the negative control group,and the non-treated cells were assigned to the blank control group.After transfection of 48h,MACC1 mRNA was detected by qRT -PCR technique,and the expression of MACC1,HGF and C -Met proteins on ovarian ectopic endometrial interstitial cells were assessed by Western blot.The proliferation ability of cells was tested by using MTT assay.The apoptosis of cells was investigated by flow cytometry methods.Results The expression of MACC1 mRNA in the lesions of ovarian endometriosis was significantly higher than that in the control group (P <0.05).The expression of MACC1 mRNA and MACC1,HGF,C -Met proteins in transfected group was significantly lower than that in negative control group and blank control group (P <0.05).Furthermore,cell proliferation activity was obviously reduced,while the apoptosis rate was significantly increased (P <0.05).Conclusion The inhibition of MACC1 gene expression can reduce the proliferation activity of ovarian ectopic endometrial interstitial cells and induce apoptosis through HGF/C-Met signaling pathway.

关 键 词：结肠癌转移相关因子-1 子宫内膜异位症 增殖 凋亡 间质细胞 

分 类 号：R711.71[医药卫生—妇产科学]