狼疮鼠肾组织白细胞介素-1β及claudin-5的表达特点及其意义的研究  

Expression and significance of interleukin-1β and claudin-5 in lupus mice renal tissue

作　　者：李楠[1] 凡慧 张穗盈 胡小倩[1] 范萍[1] 梁康礼[1] 程喜平[1] Li Nan;Fan Hui;Zhang Suiying;Hu Xiaoqian;Fan Ping;Liang Kangli;Cheng Xiping(Department of dermatology,the First' Affiliated Hospital of Guangzhou Medical University,Guangdong 510120,China)

机构地区：[1]广州医科大学附属第一医院皮肤科,510120

出　　处：《中华风湿病学杂志》2018年第11期763-767,793,共6页Chinese Journal of Rheumatology

基　　金：国家自然科学基金面上项目(81373649,81673983);广东省自然科学基金面上项目(2014A030313504).

摘　　要：目的观察炎症因子IL-1β及紧密连接蛋白claudin-5在狼疮鼠肾实质及肾膜的表达特点。方法以荧光定量PCR方法检测狼疮鼠和对照组鼠的肾膜和肾实质炎症因子IL-1β及紧密连接蛋白Claudi-5的表达特点,以免疫组织化学方法定位上述功能蛋白的表达部位。各组均数比较使用单因素方差分析法,多个样本均数之间的两两比较,方差齐时用LSD法,方差不齐时用Tamhane′sT2法。结果荧光定量PCR结果显示:炎症因子IL-1β:狼疮鼠组肾实质(0.0057±0.0013)与对照组(0.0065±0.0011)比较,差异无统计学意义(t=1.573,P>0.05);狼疮鼠组肾膜(0.0095±0.0052)较肾实质表达高,差异具有统计学意义(t=2.137,P=0.0458);狼疮鼠组肾膜IL-1β表达较对照组肾膜(0.0045±0.0033)高,差异具有统计学意义(t=2.709,P=0.0131);对照组肾实质与肾膜比较,差异无统计学意义(t=1.965,P>0.05)。claudin-5:狼疮鼠肾实质(0.0098±0.0008)与对照组(0.0116±0.0022)比较,差异无统计学意义(t=1.346,P>0.05);狼疮鼠组肾膜(0.0069±0.0001)表达低于狼疮鼠组肾实质,差异具有统计学意义(t=2.727,P=0.0173);狼疮鼠组肾膜表达低于对照组肾膜(0.0192±0.0048),差异具有统计学意义(t=6.018,P=0.0001);对照组肾膜表达高于对照组肾实质,差异具有统计学意义(t=4.009,P=0.0015)。免疫组织化学结果显示:IL-1β与claudin-5主要表达在肾小球周围及肾膜周围,肾小管很少波及。结论在狼疮鼠,IL-1β所代表的免疫炎症主要表现在血管中,肾实质表现不明显,claudin-5可受到免疫损害,但对狼疮免疫炎症从血管向组织扩散仍有屏障保护作用。Objective To study the expression difference and the meaning of interleukin (IL)-1β and Claudin-5 in the kidney and kidney's membrane between lupus mice and the control mice. Methods Gene expression difference of IL-1β and claudin-5 between lupus mice and control mice in their kidney and kidney's membrane was detected with quantitative polymerase chain reaction (Q-PCR). The location of the expression was identified by immunohistochemistry. One-way analysis of variance (ANOVA) was used to compare the means of each group, pair-wise comparison was used to compare the difference between multiple sample means. LSD method was used when the variance was equal, and Tamhane's T2 method was used when the variance was different. Results Q-PCR test results showed that IL-1β expression in lupus mice's kidney membrane (0.009 5±0.005 2) was statistically higher than lupus mice's kidney parenchyma (0.005 7±0.001 3) (t=2.137, P=0.045 8) and control mice's kidney membrane (0.004 5±0.003 3) (t=2.709, P=0.0131), however, there's no statistical significant difference between control mice's kidney membrane and parenchyma (0.006 5±0.001 1) (P>0.05), and there's no statistical difference between control and lupus mice's kidney parenchyma(P>0.05). Claudin-5 expression was statistically higher in control mice kidney membrane (0.019 2±0.004 8) than its kidney parenchyma (0.011 56±0.002 190)(t=4.009, P=0.001 5) but statistically lower in lupus mice kidney membrane (0.006 9±0.000 4) than its kidney parenchyma (0.009 8±0.002 7) (t=2.727, P=0.0173); there's no statistical significant difference between control mice's kidney parenchyma and lupus mice's kidney parenchyma (P>0.05), and lupus mice's kidney membrane expression was statistically lower than control mice's kidney membrane (t=6.018, P=0.000 1). Immun-ohistochemistry showed that IL-1β expression was mainly around glomerulus and membrane, but not renal tu-bule. Claudin-5 expression was mainly around glomerulus and membrane. Conclusion Immune inflammation induced by IL-1β has

关 键 词：狼疮肾炎 白细胞介素1Β CLAUDIN-5 组织屏障 

分 类 号：R593.241[医药卫生—内科学]