组蛋白去乙酰化酶8在干燥综合征唇腺B细胞和浆细胞中异常表达  

The abnormal expression of histone deacetylase 8 in labial gland B cells and plasma cells of Sjogren's syndrome

作　　者：刘军[1] 郭娟 万殊岑 张卓莉[1] 周炜[1] Liu Jun;Guo Juan;Wan Shucen;Zhang Zhuoli;Zhou Wei(Department.of Immunology and Rheumatology,Peking University First Hospital,Beijing 100034,China)

机构地区：[1]北京大学第一医院风湿免疫科,100034

出　　处：《中华风湿病学杂志》2018年第11期774-777,794,共5页Chinese Journal of Rheumatology

基　　金：国家自然科学基金(81373196).

摘　　要：目的探讨组蛋白去乙酰化酶8(HDAC8)在SS患者唇腺中的表达情况,以及是否与SS患者B细胞的异常激活有关。方法应用免疫组织化学方法检测HDAC8在SS患者(10例)唇腺组织与非SS患者(4例)唇腺组织中的表达情况,再通过免疫荧光双染证实哪类淋巴细胞表达HDAC8;HDAC8阳性细胞比例与患者免疫球蛋白水平进行Pearson相关性分析。结果与非SS患者相比,SS患者唇腺组织的淋巴细胞与导管上皮细胞均显著表达HDAC8;SS患者唇腺中,淋巴细胞聚集灶内HDAC8阳性细胞比例明显高于间质散在淋巴细胞[0.76±0.05和0.40±0.03,t=18.5,P<0.01]。SS患者唇腺中CD20^+B细胞与CD138^+浆细胞均表达HDAC8,CD4^+T细胞几乎不表达;SS患者淋巴细胞聚集灶中HDAC8阳性细胞比例与患者免疫球蛋白水平没有相关性。结论SS唇腺组织中B细胞和浆细胞均表达HDAC8,提示HDAC8可能参与SS中B细胞、浆细胞的异常激活。Objective To investigate the expression of histone deacetylase 8 (HDAC8) in the labial gland of patients with Sjogren's syndrome (SS), and whether it is related to the abnormal activation of B cells of SS patients. Methods The expression of HDAC8 was detected by immunohistochemistry in the labial glands from SS patients (n=10) and non-SS patients (n=4). The subtype of lymphocytes which expressed HDAC8 was determined by double staining immunofluorescence. Person correlation analyses were performed to evaluate the relation between the proportion of HDAC8 positive cells and the level of immunoglobulins in SS patients. Results The expression of HDAC8 in the labial gland from SS patients was significantly higher than that in the non-SS patients.The proportion of HDAC8 positive cells in lymphocytic foci was higher than that in scattered interstitial lymphocytes [(0.76±0.05) vs (0.40±0.03), t=18.5, P<0.01]. IF showed that HDAC8 was expressed in CD20^+ B cells, and CD138^+ plasma cells in labial gland from SS group, but not CD4^+ T cells. The ratio of HDAC8+ cell in infiltrating lymphocytic loci was not related to the level of IgG or IgA of SS patients. Conclusion HDAC8 is expressed in CD20^+ B cells and CD138^+ plasma cells in labial gland from SS patients. These data suggests that HDAC8 may be involved in abnormally activated B cells and plasma cells in SS patients.

关 键 词：干燥综合征 组蛋白去乙酰化酶 B细胞 自身免疫 

分 类 号：R593.2[医药卫生—内科学]