鼻腔填塞材料对人鼻腔黏膜上皮纤毛活性的影响  

The effect of the nasal packing material on nasal epithelial cells

作　　者：杨国慧 韩德民 臧洪瑞 王彤 李立峰 YANG Guohui;HAN Detain;ZANG Hongrui;WANG Tong;LI Lifeng(Department of Otolaryngology Head and Neck Surgery,Beijing Tongren Hospital,Capital Medical University,Beijing Institute of Otolaryngology,Key Laboratory of Otolaryngology Head and Neck Surgery (Capital Medical University),Ministry of Education,Beijing,100730, China)

机构地区：[1]首都医科大学附属北京同仁医院,北京市耳鼻咽喉科研究所,耳鼻咽喉头颈科学教育部重点实验室(首都医科大学),北京100730

出　　处：《临床耳鼻咽喉头颈外科杂志》2018年第24期1900-1903,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：北京市医院管理局“使命”人才计划项目(No:SML20150201).

摘　　要：目的:探究聚乙二醇双丙烯酸酯复合物水凝胶降解物质对鼻黏膜纤毛传输系统的影响。方法:实验根据不同配比将聚乙二醇双丙烯酸酯、壳聚糖、聚乙烯醇材料通过光交联形成复合物水凝胶,根据国际通行医疗器械生物学评价标准将聚乙二醇双丙烯酸酯复合物水凝胶材料、明胶海绵材料、壳聚糖材料配制成浸提液。实验分为聚乙二醇双丙烯酸酯复合物水凝胶材料组(A组)、明胶海绵材料组(B组)、壳聚糖材料组(C组)及对照组(D组),因各填塞材料用DMEM/F12∶BEGM培养液浸提,故选用DMEM/F12∶BEGM培养液作对照。取人鼻腔钩突组织,经气液界面培养。高速数字显微视频成像系统检测纤毛摆动频率,分别测量加药前基础状态(0min)及加药后1h、1d、2d、3d内的CBF。结果:(1)A、B、C、D组随着天数增加,纤毛摆动频率均呈现先增加后减慢的趋势。(2)A组与其他各组相比,纤毛摆动频率数值变化最大(P<0.05),提示聚乙二醇双丙烯酸酯复合物水凝胶材料可使纤毛摆动频率增加。(3)B、C、D组间的最大增加量与最大减少量相比,差异无统计学意义(P>0.05),提示可吸收性明胶海绵材料和可吸收性壳聚糖材料对鼻黏膜纤毛活性无影响。结论:聚乙二醇双丙烯酸酯复合物水凝胶材料可使体外气液界面培养的鼻黏膜上皮细胞纤毛摆动频率增加,从而使鼻黏膜纤毛活性增强,因此可以满足该新材料临床应用的安全性评价需求。Objective:The aim of this study is to investigate the effect of polyethylene glycol acrylate hybrid hydrogel degradation substance on nasal mucociliary transport system.Method:In our experiment,the complex hydrogel were formed by the combination of different ratios of polyethyleneglycol diacrylate,chitosan and polyvinyl alcohol.The experiment was divided into four groups according to the international standard of medical devices.DMEM/F12∶BEGM culture medium was used for each packing material extraction,DMEM/F12∶BEGM culture medium was used as control.Human nasal uncinate tissue,gas liquid interface culture of human nasal mucociliary epithelium cells.The high frequency digital microscope video imaging system was used to detect the ciliary wiggle frequency.The baseline state(0 min)and 1 h,1 d,2 d,3 dof CBF were measured after dosing.Result:(1)In the experimental group and the control group,with the increase of the number of days,the ciliary beat frequency increased first and then slowed down.(2)Compared with the other groups,Polyethylene glycol acrylate hybrid hydrogel group increase on CBF(P<0.05).There was no significant difference between the maximum increase and the maximum decrease in B,C and D groups(P>0.05).Therefore,it can be concluded that absorbable gelatin sponge material and absorbable chitosan material had no effect on nasal mucociliary activity.Conclusion:Polyethylene glycol diacrylate composite hydrogel material can increase the frequency of ciliary wounded nasal mucosa epithelial cells cultured in air-liquid interface in vitro,and enhanced the activity of nasal mucosa cilia.Therefore,it can meet the safety requirements of clinical application of the new material.

关 键 词：聚乙二醇双丙烯酸酯水凝胶 上皮细胞 纤毛摆动频率 体外气液界面培养 

分 类 号：R765[医药卫生—耳鼻咽喉科]