柴胡与其内生真菌共培养的内生真菌中柴胡总皂苷含量测定  

Determination of the content of saikosaponins of Bupleurum scorzonerifolium Willd. and endophytic fungi co-cultured with its endophytic fungi

作　　者：程玉鹏[1,2] 王洪月[1] 高宁[1] 宁愿 CHENG Yu-peng;WANG Hong-yue;GAO Ning;NING Yuan(School of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin 150040,China;School of Life Science and Technology,Harbin Normal University,Harbin 150025,China)

机构地区：[1]黑龙江中医药大学药学院,黑龙江哈尔滨150040 [2]哈尔滨师范大学生命科学与技术学院,黑龙江哈尔滨150025

出　　处：《化学工程师》2018年第12期19-21,共3页Chemical Engineer

基　　金：国家自然科学基金项目(81573539);黑龙江中医药大学研究生创新科研项目(yjscx2017054)

摘　　要：建立共培养的狭叶柴胡内生真菌中总皂苷的含量测定方法。方法:采用紫外可见分光光度法,以柴胡皂苷d为对照品,在波长543nm处对样品中的总皂苷进行含量测定。结果:柴胡总皂苷在0.03256～0.16280mg有良好的线性关系(r=0.9996),平均回收率为99.72%,RSD为1.21%(n=6),重复性RSD=1.32%,精密度RSD=0.41%。结论:所建立的方法准确、简便,重现性好,适用于柴胡内生真菌中总皂苷的含量测定。Objective:To establish the method for determination of the content of saikosaponins in endophytic fungi of Bupleurum scorzonerifolium Willd.with co-cultured.Methods:Saikosaponin d was used as the chemical reference substance determine the content of saikosaponins by UV-Visible Spectrophotometry.The absorbency was measured at 543nm.Results:There was good linearity in the range of 0.03256~0.16280 mg(r=0.9996)for saikosaponins.The average recovery was 99.72%with RSD=1.21%(n=6),repetition RSD=1.20%and precision RSD=0.34%.Conclusion:The method was proved to be precise,simple and reproduciable.It can be used for the quantitative determination of saikosaponins in endophytic fungi of Bupleurum scorzonerifolium Willd.

关 键 词：紫外可见分光光度法 狭叶柴胡 内生真菌 共培养 总皂苷 含量测定 

分 类 号：R927.2[医药卫生—药学]