温度、光照对微小亚历山大藻Z1产毒含量和组成的影响  

Effects of Temperature and Light Intensity on Growth and Toxin Productivity of Alexandrium minutum Z1

作　　者：余新威[2] 陈雨涔 王亚军 杜若童 方力[2] 罗红宇 YU Xinwei;CHEN Yucen;WANG Yajun;DU Ruotong;FANG Li;LUO Hongyu(key Laboratory of the Key Techniques on Health Hazard Factors of Seafood in Zhejiang Province, Institute of Food and Pharmacy,Zhejiang Ocean University,Zhoushan,Zhejinag 316022,China;Zhoushan Center for Disease Control and Prevention,ZhouShan,Zhejiang 316021,China)

机构地区：[1]浙江省海产品健康危害因素关键技术研究重点实验室,浙江海洋大学食品与医药学院,浙江舟山316022 [2]浙江省舟山市疾病预防控制中心,浙江舟山316021

出　　处：《海洋湖沼通报》2018年第5期66-71,共6页Transactions of Oceanology and Limnology

基　　金：2015国家星火计划项目(2015GA700094);2015年浙江省大学生科技创新活动计划暨新苗人才计划项目(2015R411059)资助.

摘　　要：本实验在试图通过一株微小亚历山大藻Z1(AlexandriumminutumZ1)来生产和制备膝沟藻毒素(gonyautoxin GTX)的过程中,利用不同温度、光照强度对Z1进行批次培养,采用浮游植物计数板法和高效液相色谱串联质谱检测法(HPLC-MS)监测生长状况和检测毒素的含量和组成,探索适宜的生长和产毒条件。结果表明,微小亚历山大藻Z1在15~30℃的温度范围内和15.6~125μEm-2s-1光强范围内都可生长和增殖,最适生长温度为25℃,最适生长光强为62.5~125μEm-2s-1。随着培养时间的延长,毒素含量出现先增加后减少的趋势,最大值出现在平稳期,而毒素中GTX1/4占总毒素的摩尔含量比不断上升,相应地GTX2/3的占比不断下降。适中的温度(25℃)和光照强度(62.5μEm-2s-1)都可以促进Z1的毒素总含量及GTX1/4占总毒素的摩尔含量。掌握这些产毒规律,为利用微小亚历山大藻在生产麻痹性贝毒提供理论指导。In attempting to produce and prepare gonyautoxin(GTX)by Alexandrium minutum Z1,we tested the effects of different temperature and light intensity on the growth and toxin productivity of Z1 in the batch culture with the methods of phytoplankton count plate method and high performance liquid chromatography tandem mass spectrometry assay(HPLC-MS)in order to find the suitable growth and toxin-producing conditions.Results showed that the Alexandrium minutum Z1 was able to grow and divide at temperatures varying between 15 and 30℃and under light intensity ranging from 15.6 to 125μEm-2 s-1.The optimum temperature for growth was 25℃and the optimum light intensity for growth was 62.5~125μEm-2 s-1.With the extension of incubation time,the toxin content first increased and then decreased,peaked in stationary phase.With the extension of time,the proportion of GTX1/4 increased and that of GTX2/3 dropped accordingly.Moderate temperature(25℃)and light intensity(62.5μEm-2 s-1)were able to promote the toxin content of Z1.This understanding could be mastered to provide a theoretical guidance for the production of paralytic shellfish toxins by Alexander minutum.

关 键 词：微小亚历山大藻Z1(Alexandriumminutum) 膝沟藻毒素 温度 培养时间 光照强度 毒素制备 

分 类 号：Q[生物学]