栉孔扇贝胚胎和幼虫中的17β-HSD8表达分析  

Expression Analysis of 17β-Hsd8 in Embryos and Larvae of Chlamys Farreri

作　　者：季爱昌 刘建国[1] 刘丹雯 张志峰[1] JI Aichang;LIU Jianguo;LIU Danwen;ZHANG Zhifeng(Key Laboratory of Marine Genetics and Breeding(Ocean University of China), Ministry of Education,Qingdao 266003)

机构地区：[1]中国海洋大学海洋生物遗传育种教育部重点实验室,山东青岛266003

出　　处：《海洋湖沼通报》2018年第5期125-129,共5页Transactions of Oceanology and Limnology

基　　金：国家"863"高技术研究发展计划(2012AA10A402)资助

摘　　要：17β-羟类固醇脱氢酶8(17β-HSD8)广泛分布在脊椎动物体内的多种组织中,调节类固醇激素代谢和脂肪酸代谢。本文采用荧光定量RT-PCR和原位杂交技术,对栉孔扇贝胚胎和幼虫中17β-HSD8mRNA的时空表达进行了分析。研究结果表明,栉孔扇贝17β-HSD8在未受精卵中高水平表达,受精卵和卵裂期胚胎中表达量显著下降,囊胚中的表达量显著提高。提示栉孔扇贝17β-HSD8是一个母源表达的基因,其合子基因在囊胚时期开始表达。原位杂交结果显示,17β-HSD8mRNA阳性信号在各发育阶段的胚胎和担轮幼虫中均匀分布,在D形幼虫中主要定位在内脏团附近,提示17β-HSD8可能参与起始器官发生。17β-hydroxysteroid dehydrogenases 8(17β-HSD8)is widely expressed in various tissues of vertebrates and regulates steroid biosynthesis and fatty acid metabolism.In this study,temporal and spatial expression patterns of 17β-HSD8 mRNA in embryos and larvae of the scallop Chalyms farreri were investigated using RT-PCR and in situ hybridization techniques.Abundance of the 17β-HSD8 mRNA was highly in unfertilized egg,decreased significantly in fertilized eggs and embryos at cleavage stage,and increased sharply in blastula.These suggested a maternal expression gene and the zygotic expression of 17β-HSD8 started at blastula stage.In situ hybridization detection revealed that the positive signals of 17β-HSD8 mRNA were evenly distributed in cells of embryos at various stages.In the Dshaped larva,the positive signals gathered around the visceral mass,implying apotential role of 17β-HSD8 in initiation of organgenesis.

关 键 词：栉孔扇贝 17β-HSD8 早期发育 胚胎和幼虫 

分 类 号：Q751[生物学—分子生物学]