小半夏汤对化疗致胃肠黏膜炎模型大鼠胃肠组织NF-κB、IL-1β、TNF-α mRNA和蛋白表达水平的影响  

Effect of Xiaobanxia Decoction on NF-κB,IL-1β,TNF-α mRNA and Protein Expression Levels in Sinuses Ventriculi and Ileum of Gastrointestinal Mucositis Model Rats Induced by Chemotherapy

作　　者：杜静[1] 张启龙[1] 李贵生[1] 聂克 DU Jing;ZHANG Qilong;LI Guisheng;NIE Ke(Shandong University of Tranditional Chinese Medicine,Jinan 250355,China;Guangdong Pharmaceutical University,Guangzhou 510006,China)

机构地区：[1]山东中医药大学,山东济南250355 [2]广东药科大学,广东广州510006

出　　处：《山东中医杂志》2018年第12期1020-1026,共7页Shandong Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(编号:81373828;81673779);山东省高等学校科技计划项目(编号:J16LK07)

摘　　要：目的:观察小半夏汤对顺铂化疗致胃肠黏膜炎模型大鼠胃窦和回肠组织中核因子κB(NF-κB)、白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α) mRNA和蛋白表达水平的影响,探讨小半夏汤防治化疗性胃肠黏膜炎的机制。方法:雄性Wistar大鼠112只随机分为7组,每组16只。空白对照组和顺铂模型组每日予20 mL/kg蒸馏水灌胃,小半夏汤正常对照组每日予1.6 g/kg小半夏汤灌胃,奥美拉唑组每日予2.4 mg/kg奥美拉唑灌胃,小半夏汤低、中、高剂量组每日分别予0.8 g/kg、1.6 g/kg、3.2 g/kg小半夏汤灌胃。各组上述剂量分早、晚两次给药,连续给药5 d。给药第3天,除空白对照组和小半夏汤正常对照组腹腔注射等容积生理盐水,其他各组均腹腔注射顺铂6 mg/kg造模。造模后24 h和72 h取各组大鼠胃窦和回肠,采用RT-PCR、Western blot法检测胃肠组织中NF-κB、IL-1β、TNF-αmRNA和蛋白表达水平。结果:造模后24 h和72 h,与空白对照组比较,顺铂模型组大鼠胃窦和回肠组织中NF-κB、IL-1β、TNF-α的mRNA表达水平均显著升高(P<0.01或P<0.001);同时间点小半夏汤高剂量组胃窦和回肠组织中NF-κB、IL-1β、TNF-α的mRNA表达水平显著低于顺铂模型组(P<0.01或P<0.001)。造模后24 h和72 h,顺铂模型组大鼠胃窦组织中NF-κB、IL-1β、TNF-α的蛋白表达水平均显著升高(P<0.01或P<0.001),回肠组织中除TNF-α蛋白表达水平无明显变化外,NF-κB和IL-1β的蛋白表达水平亦显著升高(P<0.001);同时间点小半夏汤各剂量组大鼠胃窦和回肠组织中NF-κB、IL-1β、TNF-α的蛋白表达均不同程度低于顺铂模型组。结论:小半夏汤防治化疗性胃肠黏膜炎的机制可能与抑制胃肠道炎性因子表达有关。Objective:To observe the effect of Xiaobanxia Decoction(XBXD) on nuclear factor-κB(NF-κB),interleukin-1β(IL-1β),tumor necrosis factor-ɑ(TNF-α) mRNA and protein expression levels in sinuses ventriculi and ileum of gastrointestinal mucositis model rats induced by cisplatin,so as to explore the mechanism of XBXD in preventing and treating gastrointestinal mucositis induced by chemotherapy. Methods:A total of112 male Wistar rats were randomly divided into control group,XBXD normal control group,cisplatin model group,omeprazole group,XBXD low dose group,XBXD middle dose group and XBXD high dose group,with 16 rats in each group. The rats in the control group and cisplatin model group were given distilled water 20 mL/kg a day by oral gavage. The rats in the XBXD normal control group were given XBXD1.6 g/kg a day by oral gavage. The rats in the omeprazole group were given omeprazole 2.4 mg/kg a day by oral gavage. The rats in the XBXD low,middle and high dose groups were given XBXD 0.8 g/kg,1.6 g/kg,3.2 g/kg a day by oral gavage respectively. The above dose was given to the rats at two separate time(morning and evening) and the medicines were given to the rats for 5 consecutive days. On the third day,the rats in the control group and XBXD normal control group were given equal volume of physiological saline and the rats in the other groups were given cisplatin 6 mg/kg to establish the gastrointestinal mucositis model. Twenty-four hours and seventy-two hours after modeling,the mRNA and protein expression levels of NF-κB,IL-1β,TNF-α in rats’ sinuses ventriculi and ileum were measured by PCR and Western Blot. Results:Twenty-four hours and seventy-two hours after modeling,compared with the control group,the NF-κB,IL-1β,TNF-α mRNA expression levels in rats’ sinuses ventriculi and ileum of the cisplatin model group were increased significantly(P<0.01 or P<0.001). At the same time,the NF-κB,IL-1β,TNF-α mRNA expression levels in rats’ sinuses ventriculi and ileum of the XBXD high dose group were significantly

关 键 词：小半夏汤 化疗性胃肠黏膜炎 核因子ΚB 白介素-1Β 肿瘤坏死因子-α 

分 类 号：R285.5[医药卫生—中药学]