大黄胶囊对糖尿病大鼠视网膜血管保护作用的研究  

Effect of Dahuang Zhechong Pills(大黄虫胶囊) on Retinal Capillary Pericytes Apoptosis in Diabetic Rats

作　　者：郭承伟[1] 朱晓林[1] 郭超红 宁云红[1] 田丽珍[2] 吕璐[2] GUO Cheng-wei;ZHU Xiao-lin;GUO Chao-hong;NING Yun-hong;TIAN Li-zhen;LYU Lu(Shandong University of Traditional Chinese Medicine,Ji'nan Shandong 250011,China;The Affiliated Hospital of Shandong University of Chinese Medicine,Ji'nan Shandong 250011,China)

机构地区：[1]山东中医药大学,山东济南250011 [2]山东中医药大学附属医院,山东济南250011

出　　处：《中医药导报》2018年第24期88-91,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(81473734)

摘　　要：目的:观察大黄胶囊对糖尿病大鼠视网膜毛细血管及周细胞形态学的影响。方法:SPF级Wistar雄性大鼠50只,随机分为空白组、模型组、大黄胶囊低、中、高剂量组,每组10只。采用链脲佐菌素(STZ)诱导糖尿病大鼠模型成功后,分别于造模后6周和12周,通过视网膜消化铺片联合PAS染色和透射电镜,观察糖尿病大鼠视网膜毛细血管和周细胞形态学改变。结果:(1)血管形态观察:造模6周,模型组视网膜毛细血管排列较为紊乱;大黄胶囊低剂量组血管偶见扭曲,中、高剂量组血管分布较规则;各组间动静脉管径比值比较,差异无统计学意义。12周时,模型组视网膜毛细血管结攀,排列紊乱明显,可见微血管瘤;大黄胶囊低剂量组血管分布欠规则,中、高剂量组血管分布较规则,无明显扭曲;各组间动静脉管径比值比较,差异有统计学意义。(2)细胞形态学观察:造模6周,除模型组视网膜毛细血管周细胞数量轻度减少外,其余各组均未见明显变化,各组间E/P值比较,差异无统计学意义。造模12周,除空白组外,各组均见不同程度周细胞数量减少,尤以模型组与大鼠胶囊低剂量组明显,并见影周细胞;与模型组比较,各组E/P值比较,差异有统计学意义。(3)透射电镜观察:造模12周,除空白组外,各组视网膜毛细血管基膜不同程度增厚,周细胞肿胀、染色质分布不均,但大鼠胶囊中、高剂量组明显好于模型组及低剂量组。结论:大黄胶囊能减轻糖尿病大鼠视网膜毛细血管及周细胞的损伤,对糖尿病视网膜微血管具有较好的保护作用。Objective:To investigate the effects of Dahuang Zhechong pills (大黄■虫胶囊)on retinal capillary pericytes apoptosis in the pathologic progression of diabetic retinopathy in rats.Methods:The effect of Dahuang Zhechong pills on diabetic retinopathy was examined in streptozotocin (STZ)-induced diabetic rat model. Rats that received STZ injection were randomized into four groups after onset of diabetes.The grouped diabetic rats were fed chow diet alone (diabetic control)or chow diet containing low dose (LD),medium dose (MD)or high dose (HD)of the pills for 6 or 1 2weeks.Rats received blank injection were fed chow diet throughout the study and used as non-diabetic control.The retinas collected at week 6 and 12 were subjected to Transmission Electron Microscope (TEM)and Periodic acid-Schiff (PAS)stain for histopathological and morphological characterization.Results:At 6 week,diabetic control rats fed chow diet alone developed capillaropathy in the form of vessels distortion and capillary wall thickening compared to the healthy non-diabetic counterparts.Comparatively, diabetic rats fed LD pills presented more regular capillary network despite few capillary distortions and those fed MD and HD pill presented uniform vessel layers.The diabetic microvascular changes became more notice-able at week 12 in diabetic control group,represented by increasing vascular tortuosity and formation of microaneuirysm.In contrast,rats from LD group showed uniform capillary wall thickness despite the presence of partial disrupted vascular network.Moreover,the MD and HD pills treatment prevented the diabetic capillary damages. Pericytes loss was found in diabetic control rats at week 6,which became more confound at week 12 accompanied with the presence of ghost cells.Whereas the diabetic rats fed pills was prevented from pericytes death at week 6 and presented dose-dependent reduction in pericyte loss and ghost ceils number at week 12.Consistently,the TEM results showed that pill treatment for 12 weeks improved the diabetic uhrastruc

关 键 词：大黄■虫胶囊 糖尿病 视网膜毛细血管 周细胞 细胞形态学 大鼠 

分 类 号：R285.5[医药卫生—中药学]