牛蓝舌病病毒VP7抗体胶体金试纸条检测方法的建立  

Establishment of colloidal gold strip for the detection of antibodies against bluetongue virus in bovine

作　　者：王慧煜[1] 刘亚东 梅琳[1] 艾军 孔玉方 韩雪清[1] WANG Hui-yu;LIU Ya-dong;MEI Lin;AI Jun;KONG Yu-fang;HAN Xue-qing(Chinese A cademy of Inspection and Quarantine Sciences ,Beijing 100176,China;Kunming Customs, Kunming 650051,China)

机构地区：[1]中国检验检疫科学研究院,北京100176 [2]昆明海关,云南昆明650051

出　　处：《中国兽医科学》2019年第1期7-12,共6页Chinese Veterinary Science

基　　金：十三五重点研发计划项目(2016YFD0500908);公益性行业科研专项(201510017)

摘　　要：为建立一种快速检测牛蓝舌病病毒抗体的免疫层析方法,本研究通过胶体金标记蓝舌病病毒VP7蛋白,以兔抗牛IgG作为检测线,抗蓝舌病毒VP7蛋白单克隆抗体作为质控线,采用间接法制备了胶体金免疫层析试纸条。结果显示,该胶体金试纸条在蓝舌病病毒抗体阳性血清稀释度为1:64时仍可检出;该试纸条与牛结核病、牛布鲁氏菌病、牛病毒性腹泻、牛巴氏杆菌病、牛地方流行性白血病的阳性血清均无交叉反应;与国外商品化试剂盒相比较,该试纸条检测结果与ELISA检测试剂盒(IDEXX)的符合率为92.7%(51/55)。结果表明,本试验初步建立的牛蓝舌病病毒抗体的胶体金免疫层析检测方法具有较好的特异性和稳定性,整个检测过程可在10 min内完成,能够快速检测样品血清,适用于现场快速检测。To establish a colloidal gold immunochromatography test strip for rapidly detecting the antibodies against bluetongue virus in bovine,the recombinant protein VP7 was used for colloidal gold marker and the goat-cow IgG was served as test line and the VP7 monoclonal antibody was served as control line.In result,even 1:64 diluted standard bovine bluetongue disease-positive sera could be detected.The prepared colloidal gold strip showed no cross reaction with the sera with other related diseases.Compared with the foreign commercial kit,the coincidence rate between the strip and the commercial ELISA kit (IDEXX) was 92.7%(51/55).This entire test process could be completed within 10 minutes. In conclusion,the prepared colloidal gold strip showed high specificity and stability,and it might be used for the clinical detection and large-scale epidemiological survey of bluetongue virus in bovine.

关 键 词：蓝舌病病毒 VP7蛋白 单克隆抗体 胶体金 免疫层析试纸条 

分 类 号：S852.659.4[农业科学—基础兽医学]