微波消解/ICP-MS测定胶原蛋白中14种金属元素的研究  

Determination of 14 metal elements in collagen by inductively coupled plasma mass spectrometry with microwave digestion

作　　者：刘斌[1,2] 施燕平[1,2] 徐志洲 卢建 刘莉莉[1,2] Liu Bin;Shi Yanping;Xu Zhizhou;Lu Jian;Liu Lili(Shandong Quality Inspection Center for Medical Devices, Key Laboratory of Biological Evaluation of Medical Advices of Shandong Province, Ji'nan 250101, China;SFDA-Jinan Quality Supervision and Inspection Center for Medical Devices, Ji'nan 250101, China)

机构地区：[1]山东省医疗器械产品质量检验中心,山东省医疗器械生物学评价重点实验室,济南250101 [2]国家食品药品监督管理局济南医疗器械质量监督检验中心,济南250101

出　　处：《国际生物医学工程杂志》2018年第5期428-433,共6页International Journal of Biomedical Engineering

基　　金：山东省重点研发计划(2015GSF118165).

摘　　要：目的 建立测定胶原蛋白中Mn、Cu、Zn、Al、Ti、Pb、Ba、Cd、Co、Cr、Ni、Fe、As和Hg 14种金属元素的方法。方法 首先优化了胶原蛋白微波消解的条件、电感耦合等离子体质谱(ICP-MS)工作条件以及各金属元素在ICP-MS测定中采用的模式,采用微波消解/ICP-MS测定了9个不同规格型号的胶原蛋白样品中的14种金属元素含量,同时进行了加标回收验证和方法准确性测定。结果 仪器检出限为0.1~5.0 μg/L,方法检出限为0.001 25~0.625 mg/kg,测定的相对标准偏差(n=11)不大于4.0%,样品的回收率均在87.7%~107.4%。在相同条件下,对猪肝标准品中的14种金属元素进行了测定,测定值均在标准范围内。结论 本研究建立的微波消解/ICP-MS法具有快速、简便、灵敏度高、稳定性好、准确度高等优点,值得借鉴和推广。Objective To propose an analysis method for determining 14 kinds of metal elements in collagen, including Mn, Cu, Zn, Al, Ti, Pb, Ba, Cd, Co, Cr, Ni, Fe, As and Hg. Method The conditions of collagen microwave digestion, inductively coupled plasma mass spectrometry (ICP-MS) working conditions and the modes used in the determination of various metal elements in ICP-MS were optimized. The content of 14 metal elements in 9 kinds of collagens with different specifications was determined by microwave digestion/ICP-MS, and the tagged recovery verification and method accuracy determination were conducted. Results The detection limit of the instrument was 0.1~5.0 μg/L. The detection limit of the proposed method was 0.001 25~0.625 mg/kg. The relative standard deviation (n=11) was not more than 4.0%, and the recovery rate of the sample was 87.7%~107.4%. Under the same conditions, 14 metal elements in the pig liver standard were measured and the measured values were within the standard range. Conclusions The microwave digestion/ICP-MS method possesses the advantages of rapidness, simplicity, high sensitivity, good stability and high accuracy. It is worthy of reference and promotion.

关 键 词：微波消解 电感耦合等离子体质谱 胶原蛋白 金属元素 

分 类 号：R927.1[医药卫生—药学]