淫羊藿苷与BMP-2对体外培养成骨细胞增殖的影响  

Effect of Icariin and BMP-2 on proliferation of the cultured osteoblasts in vitro

作　　者：谢利娜[1] 刘艳辉[1] 赵波[1] 景向东[1] XIE Li-na;LIU Yan-hui;ZHAO Bo;JING Xiang-dong(The first Affiliated Hospital,Guangzhou University of Chinese Medicine,Guangzhou 510405,China)

机构地区：[1]广州中医药大学第一附属医院口腔科,广州510405

出　　处：《北京口腔医学》2018年第6期319-322,共4页Beijing Journal of Stomatology

基　　金：广州中医药大学青年人才培优项目.广东省医学科研基金(A2016551)

摘　　要：目的比较观察淫羊藿苷(ICA)和BMP-2分别对成骨细胞增殖的影响并探讨可能机制。方法采用h FOB1. 19人成骨细胞株体外构建人成骨细胞系,分别使用不同浓度淫羊藿苷和BMP-2干预,利用MTT法检测成骨细胞增殖率,Western blotting检测OPG和BMP-2蛋白表达,实时荧光定量RT-PCR检测成骨细胞中BMP-2、OPG、ALP mRNA的表达。结果 MTT法检测表明终浓度为10～40μg/ml的ICA和50μg/ml的BMP-2均能促进成骨细胞的增殖,Western blotting检测两者均能促进BMP-2、OPG蛋白的表达。实时荧光定量RT-PCR结果显示ICA不同程度上调BMP-2、OPG、ALP mRNA的表达,浓度为20μg/ml、30μg/ml ICA的成骨作用明显,与50μg/ml BMP-2的成骨作用相当,浓度为50μg/ml ICA对成骨细胞增殖、相关蛋白和基因表达没有明显作用。结论淫羊藿苷促进体外培养成骨细胞增殖,可能通过促进BMP-2、OPG蛋白表达和BMP-2、OPG、ALP mRNA的表达完成。一定浓度ICA与BMP-2具有等同的成骨作用。Objective To investigate the effect of icariin( ICA) and bone morphogenetic protein 2( BMP-2) on the proliferation of the cultured osteoblasts in vitro and the possible mechanism. Methods Human osteoblasts was obtained in vitro from h FOB 1. 19 osteoblast cell lines. The osteoblasts were cultured with different concentrations of ICA and BMP-2respectively. MTT test was used to observe the proliferation rate of osteoblasts. The protein expression of OPG and BMP-2was detected by Western blotting. The expression of BMP-2,OPG and ALP mRNA was examined by Real-time fluorescence quantitative RT-PCR. Results The MTT assay showed that the ICA( 10-40μg/ml) and BMP-2( 50μg/ml) could promote the proliferation of osteoblasts. Both ICA and BMP-2 could increase the expression of BMP-2 and OPG protein. Conclusion Icariine may enhance the proliferation of cultured human osteoblasts by increasing the expression of BMP-2,OPG protein,BMP-2,OPG and ALP mRNA. A certain concentration of ICA is equivalent to BMP-2 in osteogenesis.

关 键 词：淫羊藿苷 BMP-2 成骨细胞 增殖 

分 类 号：R349.6[医药卫生—基础医学]