辽宁北部某地区生活饮用水源水污染贾第鞭毛虫和隐孢子虫情况调查  

Giardia and cryptosporidium contaminations in source water in a region of northern Liaoning

作　　者：魏彤竹[1] 于淼[1] 张眉眉[1] 马景宏[1] WEI Tong -zhu;YU Miao;ZHANG Mei-mei;MA Jing -hong Liaoning(Provincial Center for Disease Control and Prevention,Shenyang,Liaoning 110005,China)

机构地区：[1]辽宁省疾病预防控制中心,辽宁沈阳110005

出　　处：《中国卫生检验杂志》2019年第2期239-241,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的检测水源水是否被贾第鞭毛虫和隐孢子虫污染。方法采用GB 5750. 12—2006中的免疫磁荧光抗体法对所采集的水样进行样品检测,同时采用巢式PCR方法对平行样品进行验证。结果本实验对采自辽宁北部某地区5处生活饮用水源水水样进行检测,其中贾第鞭毛虫检出率为60. 0%,隐孢子虫的检出率为40. 0%,2份阳性对照水样回收率符合标准要求,且PCR扩增反应结果与镜检结果一致。结论辽宁北部地区生活饮用水水源水存在被两虫污染的情况,应加强供水网络的消毒处理,防止出厂水被两虫二次污染。Objective To investigate whether source water was contaminated by giardia and eryptosporidium.Methods The samples were tested by immunomagnetic separation fluorescence anti-body method in GB 5750.12-2006,and the parallel samples were also tested by nested PCR.Results The water samples from 5 different water sources in northern Liaoning Province were tested.The detection rate of giardia was 60.0%,and that of cryptospofidium was 40.0%.The recovery of two positive control water samples met the standard requirements.The results of nested PCR were consistent with those of microscopy. Conclusion Souree water in northern Liaoning Province is polluted by giardia and cryptospofidium.,Therefore,it is neeessary to strengthen disinfeetion in water supply network so as to prevent secondary contamination by giardia and eryptosporidium.

关 键 词：贾第鞭毛虫 隐孢子虫 免疫磁珠分离 巢氏PCR 

分 类 号：R123.1[医药卫生—环境卫生学]