液相色谱-串联质谱法测定化妆品中的双酚A  

Determination of Bisphenol A in Cosmetics by LC-MS/MS

作　　者：郑荣[1] 茹歌[1] 陈静[1] 陈丹丹[1] 钟吉强[1] 王柯[1] ZHENG Rong;RU Ge;CHEN Jing;CHEN Dan-dan;ZHONG Ji-qiang;WANG Ke(Shanghai Institute for Food and Drug Control,Shanghai 201203,China)

机构地区：[1]上海市食品药品检验所,上海201203

出　　处：《香料香精化妆品》2018年第6期46-49,共4页Flavour Fragrance Cosmetics

摘　　要：为建立测定化妆品中双酚A的液相色谱-质谱法,将样品经甲醇-氨水混合溶液提取,以Waters BEH C18柱(2.1 mm×5 cm×1.7μm)为色谱柱,以甲醇为流动相A相,以含5 mmol/L醋酸铵的水溶液为流动相B相,梯度洗脱,柱温40℃,流速0.25 mL/min,以多反应离子监测模式进行监测,内标法定量。结果表明,双酚A在0.2～50 ng/mL质量浓度范围内,线性关系良好,相关系数r> 0.995,检出限为0.005μg/g,平均回收率为85.5%～104.7%,精密度为0.9%～2.0%。该方法快速简便,适用于化妆品中双酚A类物质及其衍生物的快速筛查。To establish a LC-MS/MS method for determination ofbisphenol A in cosmetics combined with internal standard quantification,the samples were extracted with a mixture solution of methanol-0.1%(mass ratio)ammonia water,a Waters reversed phase column BEH C18(2.1mm×5cm×1.7μm) was used with methanol-5mmol/L ammonium acetate solution as the mobile phase under the condition of gradient elution,the column temperature was 40℃,flow rate was 0.25mL/min,the multi-ion monitoring mode was performed,internal standard quantification was conducted.The results showed that it had a good linearity in the range of 0.2-50ng/mL(r >0.995).The spiked recoveries were 85.5% to 104.7% with the relative standard deviation of 0.9% to 2.0%.The limit of detection was 0.005μg/g.The method is simple,rapid and accurate.It is suitable for the rapid screening of bisphenol A and relative derivatives in cosmetics.

关 键 词：双酚A 化妆品 液相色谱-串联质谱法 

分 类 号：O657.63[理学—分析化学] TQ658[理学—化学]