A cold adapt and ethanol tolerant endoglucanase from a marine Bacillus subtilis  

作　　者：Dongsheng Xue Xuhao Zeng Chunjie Gong Dongqiang Lin Shanjing Yao 

机构地区：[1]Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology,Hubei University of Technology [2]Department of Chemical and Bioengineering, Zhejiang University

出　　处：《Chinese Journal of Chemical Engineering》2018年第12期2601-2606,共6页中国化学工程学报（英文版）

基　　金：Supported by the Science and Technology Research Project of Hubei Provicial Department of Education(B2017041)

摘　　要：The catalytic properties and thermodynamic kinetics of the endoglucanase from a marine Bacillus subtilis were analyzed. Optimum pH and temperature of the endoglucanase activity were 5.0 and 35 °C. The endoglucanase activity, melt point temperature was 1.13 folds(247.02 U·ml^(-1)), 2.1 °C higher(39.2 °C) in 6% ethanol solution than that(218.60 U·ml^(-1)),(37.1 °C) in free ethanol. At 40 °C–55 °C, Gibbs free energy, ΔG, and the content ofα-helix was higher in 6% ethanol solution than that in ethanol free solution. The increasing of α-helix content led to higher activity and better thermostability in ethanol solution. The cold adapt ethanol tolerant endoglucanase was valuable for bioethanol product by simultaneous saccharification and fermentation process.The catalytic properties and thermodynamic kinetics of the endoglucanase from a marine Bacillus subtilis were analyzed. Optimum pH and temperature of the endoglucanase activity were 5.0 and 35 °C. The endoglucanase activity, melt point temperature was 1.13 folds(247.02 U·ml^(-1)), 2.1 °C higher(39.2 °C) in 6% ethanol solution than that(218.60 U·ml^(-1)),(37.1 °C) in free ethanol. At 40 °C–55 °C, Gibbs free energy, ΔG, and the content ofα-helix was higher in 6% ethanol solution than that in ethanol free solution. The increasing of α-helix content led to higher activity and better thermostability in ethanol solution. The cold adapt ethanol tolerant endoglucanase was valuable for bioethanol product by simultaneous saccharification and fermentation process.

关 键 词：ENDOGLUCANASE ETHANOL TOLERANT COLD ADAPT BIOETHANOL 

分 类 号：TQ[化学工程]