不同栽培品种高良姜HPLC指纹图谱研究及指标成分含量测定  

Study on HPLC Fingerprint and Content Determination of Index Components of Different Cultivars of Alpinia officinarum Hance

作　　者：龙琴[1,2,3] 林鼎光 胡佳莉 李钟 王淑美[1,2,3] LONG Qin;LIN Ding-Guang;HU Jia-Li;LI Zhong;WANG Shu-Mei(Guangdong Pharmaceutical University,Guangzhou 510006 Guangdong,China;Chinese Medicine Quality Engineering Technology Research Center of Guangdong Province / Higher-Education Institutions,Guangzhou 510006 Guangdong,China;Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica,State Administration of Traditional Chinese Medicine,Guangzhou 510006 Guangdong,China)

机构地区：[1]广东药科大学,广东广州510006 [2]广东省(高校)中药质量工程技术研究中心,广东广州510006 [3]国家中医药管理局中药数字化质量评价技术重点研究室,广东广州510006

出　　处：《广州中医药大学学报》2019年第1期109-114,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省科技厅项目(编号:2016A020226018);中央支持地方高校项目(编号:51348000);国家中医药管理局中药标准化行动计划项目(编号:ZYBZH-Y-GD-13)

摘　　要：【目的】比较不同栽培品种高良姜(竹头姜与蜂窝姜)HPLC特征图谱,并测定3个指标成分的含量,为高良姜的品种鉴定、质量控制及优良品种的选育提供参考。【方法】采用高效液相色谱法(HPLC)测定样品指纹图谱,并对其结果进行相似度分析、聚类分析及主成分分析。同时测定二苯基庚烷A、高良姜素、山柰素的含量。【结果】不同栽培品种高良姜指纹图谱共有峰12个,但各组分比例差异显著。竹头姜与蜂窝姜之间的相似度为0.760～0.860,明显低于同品种高良姜之间的相似度。聚类分析和主成分分析均可将竹头姜与蜂窝姜样品分开。竹头姜与蜂窝姜中二苯基庚烷A、山柰素的平均含量较接近,而高良姜素的平均含量差异显著,分别为0.30%、0.98%。【结论】HPLC指纹图谱可用于区分不同栽培品种高良姜;指标成分含量测定方法可靠,重复性好,对高良姜栽培品种的鉴定、质量控制及品种选育具有指导意义和参考价值。Objective To compare the HPLC fingerprint of different cultivars of Alpinia officinarum Hance(Zhutou galangal and Fengwo galangal) and to determine the contents of their 3 index components, so as to provide a reference for the identification, quality control and breeding of excellent varieties of Alpinia officinarum Hance. Methods The fingerprints of the samples were determined by high performance liquid chromatography(HPLC), and the results were analyzed by similarity analysis, cluster analysis and principal component analysis. The contents of diphenylheptane A,galangin and kaempferid were simultaneously determined. Results There were 12 common peaks in the fingerprints of different cultivars of Alpinia officinarum Hance,but the proportions of each component were significantly different. The similarity between Zhutou galangal and Fengwo galangal was 0.760- 0.860, which was significantly lower than the similarity among the samples of the same variety of Alpinia officinarum Hance. According to the results of both of the cluster analysis and the principal component analysis, Zhutou galangal can be identified from Fengwo galangal. The average contents of diphenylheptane A and kaempferid in Zhutou galangal were close to those of Fengwo galangal, while the average content of galangin between the two cultivars was significantly different, being 0.30% , 0.98% respectively. Conclusion HPLC fingerprint can be used to distinguish different cultivars of Alpiniao officinarum Hance. The method for determination of index components is reliable and reproducible, which has guiding significance and reference value for the identification,quality control and variety breeding of Alpinia officinarum Hance.

关 键 词：高良姜 竹头姜 蜂窝姜 HPLC指纹图谱 质量控制 

分 类 号：R284.1[医药卫生—中药学]