湖南省主栽桑品种桑叶生物碱含量的测定分析  

Determination and Analysis on Alkaloids Contents in Leaves of Main Mulberry Varieties Cultivated in Hunan Province

作　　者：张俊 颜新培 李一平 黄仁志 邵元元 蒋诗梦 蒋勇兵 邹湘月 Zhang Jun;Yan Xinpei;Li Yiping;Huang Renzhi;Shao Yuanyuan;Jiang Shimeng;Jiang Yongbing;Zou Xiangyue(Sericultural Research Institute of Hunan Province,Changsha 410127,China)

机构地区：[1]湖南省蚕桑科学研究所

出　　处：《蚕业科学》2018年第6期916-922,共7页ACTA SERICOLOGICA SINICA

基　　金：国家科技支撑计划项目(No.2012BAD36B07);湖南省科技支撑计划项目(No.2015NK3054);现代农业产业技术体系建设专项(No.CARS-18)

摘　　要：以湖南省具有代表性的15个主栽桑树品种为材料,分别采用雷氏盐比色法及反相高效液相色谱法对不同品种不同叶位桑叶总生物碱和1-脱氧野尻霉素(DNJ)含量进行测定分析。结果表明,不同桑品种不同叶位间总生物碱和DNJ含量存在极显著差异(P<0.01)。总生物碱和DNJ平均含量最高的是杂交桑沙2×伦109,是含量最低的三倍体桑品种湘桑6号的3倍;各品种从上位叶到下位叶总生物碱和DNJ含量几乎趋向于依次降低,平均质量比变幅分别为1.375~4.273 mg/g和0.705~2.149 mg/g。在供试品种上位叶和中位叶间,杂交桑沙2×伦109桑叶总生物碱和DNJ含量最高,质量比分别达到5.137 mg/g和4.581 mg/g、2.612 mg/g和2.284 mg/g;育2号和嘉陵20号下位叶的总生物碱和DNJ含量最高,质量比分别达3.599 mg/g和1.823 mg/g。综合评价认为,在湖南省栽培的桑叶生物碱含量较高的桑品种中,沙2×伦109、嘉陵20号、育2号适合作为桑叶生物碱开发专用桑树优势品种。The contents of total alkaloids and 1-deoxynojimycin (DNJ)in leaves of 15 representative mulberry varieties cultivated in Hunan Province were determined and analyzed by Reinecke salt colorimetric method and reversed-phase high performance liquid chromatography (RP-HPLC).The result indicated that the contents of total alkaloids and DNJ in leaves of different mulberry varieties and of different leaf positions had extremely significant difference (P<0.01).Total alkaloids and DNJ contents in leaves of hybrid mulberry Sha 2×Lun 109 was the highest,which was 3times as much as that of the triploid mulberry Xiangsang 6.The contents of total alkaloids and DNJ tend to decrease from upper leaves to lower leaves in turn.The average mass ratio of total alkaloids and DNJ content in mulberry leaves of different leaf positions was 1.375 to 4.273 mg/g and 0.705 to 2.149 mg/g respectively.In upper leaves and middle leaves,the maximum contents of total alkaloids and DNJ were found in hybrid mulberry Sha 2×Lun 109,with the mass ratio as high as 5.137 mg/g and 4.581 mg/g,2.612 mg/g and 2.284 mg/g,respectively.In lower leaves,maximum contents of total alkaloids and DNJ were found in Yu 2 and Jialing 20,with the mass ratio as high as 3.599 mg/g and 1.823 mg/g respectively.Comprehensive evaluation analysis showed that Sha 2×Lun 109,Jialing 20 and Yu 2 had higher practical value of total alkaloids and DNJ,being appropriate special mulberry varieties for development of alkaloids from mulberry leaf.

关 键 词：桑品种 桑叶 总生物碱 1-脱氧野尻霉素(DNJ) 叶位 

分 类 号：S888.2[农业科学—特种经济动物饲养] O629.3[农业科学—畜牧兽医]