幼年暹罗鳄致病性简达气单胞菌的分离鉴定  

Isolation and identification of Aeromonas jandaei in young Siamese alligator

作　　者：蒲文渊 曾纪锋[2] 郑挺 欧秀全 杨诺 郭桂英[1] 王宇[1] 郑继平[1] PU Wen-yuan;ZENG Ji-feng;ZHENG Ting;OU Xiu-quan;YANG Nuo;GUO Gui-ying;WANG Yu;ZHENG Ji-ping(Institute of Biotechnology,Hainan University,Haikou 570228,China;College of Animal Science and Technology, Hainan University,Haikou 570228,China;Ling Shui Hua Tianlong Breeding Company,Lingshui 572435,China;The People's Government of Guangpo Town,Lingshui County,Hainan Province,Lingshni 572400,China)

机构地区：[1]海南大学生物技术学院,海南海口570228 [2]海南大学动物科技学院,海南海口570228 [3]陵水华天龙养殖有限公司,海南陵水572435 [4]海南省陵水县光坡镇人民政府,海南陵水572400

出　　处：《中国预防兽医学报》2018年第12期1181-1184,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：海南自然科学基金面上项目(20153083);海南自然科学基金面上项目(317071);海南自然科学基金创新研究团队项目(2017CXTD005);国家自然科学基金(31460699).

摘　　要：为对海南陵水地区自然发病的幼龄暹罗鳄进行病原菌分离鉴定,本研究采用形态与生理生化特性测定并结合16S r RNA基因系统发育学分析方法,对分离的LS5菌株进行鉴定;同时对分离株进行药物敏感试验及对小鼠致病性试验。结果显示,该菌呈革兰氏阴性杆菌,氧化酶等生理生化特性与简达气单胞菌基本一致;根据16S r RNA基因的系统进化及其同源性分析,可确定分离的菌株LS5与简达气单胞菌属于同一系统发育分支,其基因与FJ940830.1 Aeromonas jandaei p W23菌株同源性高达100%;LS5菌株对小鼠的半数致死量为4.1×10~5 cfu/只,具有较强致病性;该菌对庆大霉素、丁胺卡那霉素、环丙沙星敏感,对阿莫西林、利福平、氨苄西林等抗生素耐药。本研究从海南发病幼鳄体内首次发现简达气单胞菌,同时检测到其具有较强致病性,通过药敏试验为该病的防治奠定基础。A pathogenic bacteria,designated LS5,were isolated fi:om the young Siamese crocodile in Lingshui area of Hainan province and identified by morphological,physiological and biochemical characteristics combined with the 16S rRNA phylogenetic analysis.At the same time,the isolate was tested for drug susceptibility and pathogenicity in mice.The results showed that the bacteria was gram-negative bacilli,oxidase and other physiological and biochemical characteristics were basically consistent with that of Aeromonas.According to the phylogenetic and homology analysis of the 16S rRNA gene,the isolate LS5 and Aeromonas were identified as the same phylogenetic branch,and the homology was 100%to A.jandaei PW23strain.The LDs0of the isolate LS5was 4.1×105cfu,indicating the isolate was a strong pathogenic to mice.The isolate was sensitive to gentamicin,amikacin, ciprofloxacin,and resistance to amoxicillin,rifampicin and ampicillin and other antibiotics.In this study,A.jandaei was first found in the infected young Siamese alligator in Hainan,which laid a foundation for the prevention and treatment of the related disease.

关 键 词：暹罗鳄 简达气单胞菌 分离鉴定 

分 类 号：S852.61[农业科学—基础兽医学]