冬虫夏草中一株内生菌次级代谢产物的化学成分  

Chemical constituents of one endophytic fungus isolated from Cordyceps sinensis

作　　者：邱玲[1] 何鑫 冯丹[1] 戴宇[1] 杨岚 郭大乐[1] 邓赟[1] QIU Ling;HE Xin;FENG Dan;DAI Yu;YANG Lan;GUO Dale;DENG Yun(Key Laboratory of Standardization of Chinese Herbal Medicine,Ministry of Education,State Key Laboratory Breeding of Systematic Research Development and Utilization of Chinese Medical Resources,Co-founded by Sichuan Province and MOST,Pharmacy Department,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)

机构地区：[1]成都中医药大学药学院,中药材标准化教育部重点实验室,四川省中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地,成都611137

出　　处：《应用与环境生物学报》2018年第6期1290-1294,共5页Chinese Journal of Applied and Environmental Biology

基　　金：四川省科技厅省青年科技创新研究团队专项计划(2016TD0006);四川省教育厅科研项目(18ZA0191);成都中医药大学青年教师创新基金(ZRQN1606)资助.

摘　　要：对一株冬虫夏草内生真菌Penicillium herquei发酵液次级代谢产物乙酸乙酯萃取部位的化学成分进行研究.利用柱色谱、制备色谱等手段进行分离纯化,并利用NMR、ESI-MS等波谱手段对化合物进行结构鉴定.分离了11个化合物,鉴定为2′,4′-二羟基-3′,5′-二甲基苯乙酮(1),香草醛(2),3-氧代-α-紫罗兰酮(3),3,3,5-三甲基-4-(3-氧代-1-丁烯基)-1-环己酮(4),对羟基苯甲醛(5),对羟基苯乙酮(6),对羟基苯乙酸甲酯(7),环(脯氨酸-异亮氨酸)(8),环(脯氨酸-缬氨酸)(9),4-甲氧基苯乙酸(10),环(4-甲基脯氨酸-缬氨酸)(11).本研究表明苯甲醛类、紫罗兰酮类和环二肽类化合物为冬虫夏草内生菌P. herquei的主要次级代谢产物.The study focused on the analysis of the chemical constituents of ethyl acetate extracts of Penicillium herquei, an endophyte of Cordyceps sinensis. The compounds were isolated and purified by chromatographic procedures and identified on the basis of spectral methods. Eleven compounds were identified: 2′,4′-dihydroxy-3′,5′-dimethylacetophenone (1), vanillin (2), 3-oxo-α-ionone (3), 3,3,5-trimethyl-4-(3-oxo-1-butenyl)cyclohexan-1-one (4), p-hydroxybenzaldehyde (5), p-hydroxyacetophenone (6), methyl p-hydroxyphenylacetate (7), cyclo-(Pro-Ile) (8), cyclo-(Pro-Val) (9), 4-methoxyphenylacetic acid (10), and cyclo-(4-methyl-Pro-Val) (11). Benzaldehydes, ionones, and cyclodipeptides are the major metabolites of the P. herquei from C. sinensis.

关 键 词：冬虫夏草 PENICILLIUM herquei 苯甲醛 紫罗兰酮 环二肽 

分 类 号：Q936[生物学—微生物学]