霍山石斛对肾阴虚小鼠抗疲劳及能量代谢的影响  

Effects of Dendrobium huoshanense on fatigue and energy metabolism in mice with kidney-yin deficiency

作　　者：侯燕[1] 费文婷 王玉杰[1] 周雪[1] 乐娜 王林元[1] 张建军[1] Hou Yan;Fei Wenting;Wang Yujie;Zhou Xue;Yue Na;Wang Linyuan;Zhang Jianjun(Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学,北京100029

出　　处：《北京中医药大学学报》2018年第12期995-1001,共7页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.81173569);北京中医药大学在读研究生自主课题(No.2018-JYB-XS20)~~

摘　　要：目的研究霍山石斛对肾阴虚小鼠抗疲劳及能量代谢的影响。方法采用灌服甲状腺素建立小鼠肾阴虚模型,观察霍山石斛对肾阴虚小鼠阴虚状态的影响。检测各组小鼠温度趋向性和负重游泳时间的变化,酶联免疫吸附法测定小鼠血清三碘甲状腺原氨酸(T3),甲状腺素(T4)。放射免疫分析法检测血清乳酸脱氢酶(LDH),肌酸激酶(CK),环磷酸腺苷(cAMP),环磷酸鸟苷(cGMP),计算cAMP/cGMP比值。比色法检测肝组织Na^+-K^+-ATP酶和Ca^(2+)-Mg^(2+)-ATP酶的含量。结果与正常组相比,模型组小鼠热区停留比例和负重游泳时间明显下降(P <0. 01),T3、T4、cAMP和cAMP/cGMP的含量明显升高(P <0. 01、P <0. 001),LDH显著降低(P <0. 05); CK、Na^+-K^+-ATP酶和Ca^(2+)Mg^(2+)-ATP酶显著增高(P <0. 001)。与模型组相比,霍山石斛A、B、C、D 4个剂量组和铁皮石斛组、六味地黄丸组小鼠的热区停留比例和负重游泳时间明显增高(P <0. 01、P <0. 05、P <0. 001),cAMP/cGMP明显降低(P <0. 05、P <0. 01),Na^+-K^+-ATP酶和Ca^(2+)-Mg^(2+)-ATP酶明显降低(P <0. 01、P <0. 001),霍山石斛B、C、D剂量组和铁皮石斛组、六味地黄丸组T3、T4显著降低(P <0. 05、P <001、P <0. 001),霍山石斛B、C剂量组和铁皮石斛组、六味地黄丸组的LDH显著增高,CK显著降低(P <0. 01、P <0. 05、P <0. 001)。结论霍山石斛具有滋阴清热的作用,能降低甲状腺素诱导的肾阴虚小鼠的代谢酶活性和甲状腺素水平,调节能量代谢速率,提高阴虚小鼠的负重游泳时间,具有抗疲劳作用,其机制与调节能量代谢酶作用相关,进一步验证了霍山石斛药性偏寒。Objective To explore the effects of Huoshan Shihu (Dendrobium huoshanense,HSSH)on fatigue and energy metabolism regulation in mice with kidney-yin deficiency.Methods Mice model with kidney-yin deficiency was established by garage of thyroxine.Effects of Huoshan Shihu on mice with kidney-yin deficiency were observed.The thermotropism behavior and the weight-loaded swimming time of mice were monitored.The levels of T3 and T4 were detected by using Enzyme-linked Immunosorbent Assay (ELISA);cAMP,cGMP,LDH and CK in serum were detected with radioimmunoassay,cAMP/ cGMP was thns calculated,and the Na^+-K^+-ATP-ase and Ca^2+-Mg^2+-ATP-ase in liver tissue of mice were detected with chromatometry.Results Compared with the normal animals,the time duration in high-temperature zone and the loaded-swimming time of mice in model group were significantly decreased (P <0.01).The levels of 33,T4,cAMP,and cAMP/cGMP were significantly increased (P <0.01,P <0.001).The activities of LDH were decreased (P <0.05),and CK,Na^+-K^+-ATP-ase and Ca^2+- Mg^2+-ATP-ase were increased (P <0.001).Compared with the model animals,time duration in hightemperature zone and loaded-swimming time in A (0.33g/kg),B (1g/kg),C (2g/kg),and D (3g/ kg)dose of HSSH group,Tiepi Shihu (Dendrobium candidum,TPSH)group,and Liuwei Dihuang Wan (Six-Ingredient Rehmannia pill,LWDH )group increased significantly;the level of cAMP/cGMP decreased significantly (P<0.05,P<0.01);and the activities of Na^+-K^+-ATP-ase and Ca^2+-Mg^2+- ATP-ase decreased significantly (P<0.01,P<0.001).B,C,and D dose of HSSH group,TPSH group and LWDH group appeared to reduee the concentration of T3and T4(P<0.05,P<0.01,P<0.001). the activities of LDH were significantly increased in B and C dose of HSSH,TPSH and LWDH groups, and the levels of CK decreased (P<0.05,P<0.01,P<0.001).Conclusion With the effects of nourishing yin and clearing heat,Huoshan Shihu could regulate the energy metabolism rate,improve the loaded-swimming time,and reduce the activity of energy metabolizing enzymes and the thyroxi

关 键 词：霍山石斛 抗疲劳 肾阴虚 能量代谢 小鼠 

分 类 号：R285.5[医药卫生—中药学]