弓形虫感染孕妇血清多肽指纹图谱分析及临床初步应用  

Analysis of sera peptide fingerprints from pregnant women with toxoplasmosis and preliminary clinical use of that technique

作　　者：仇志琴[1] 沈晔 唐凤[3,4] 华海涌 虞丰[1] 黄玉政 QIU Zhi-Qin;SHEN Ye;TANG Feng;HUA Haiyong;YU Feng;HUANG Yuzheng(Gynecology and Obstetrics,Wuxi Second Hospital Affiliated with Nanjing Medical University,Wuxi ,Jiangsu,China 214002;Wuxi Maternity and Child Health Care Hospital Affiliated with Nanjing Medical University, Wuxi ,Jiangsu,China 214002;Jiangsu Institute of Parasitic Diseases,National Health Commission Key Laboratory of Parasitic Disease Control and Prevention,Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology,Wuxi ,Jiangsu,China 214064;Public Health Research Center,Jiangnan University ,Wuxi ,Jiangsu, China 214122)

机构地区：[1]南京医科大学附属无锡第二人民医院妇产科,江苏无锡214002 [2]南京医科大学附属无锡妇幼保健院 [3]江苏省血吸虫病防治研究所国家卫生健康委员会寄生虫病预防与控制技术重点实验室,江苏省寄生虫与媒介控制技术重点实验室 [4]江南大学公共卫生研究中心

出　　处：《中国病原生物学杂志》2018年第12期1393-1396,共4页Journal of Pathogen Biology

基　　金：国家自然科学基金项目(No.81673673,31201893);江苏省自然科学基金项目(No.BK2011164);江苏省卫生科研项目(No.X201416,X200736,X201110).

摘　　要：目的建立孕妇弓形虫感染的辅助诊断新方法,并筛选弓形虫感染血清标志物。方法利用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)结合临床ClinProTool分析,将弓形虫IgG抗体阳性组与健康对照人群组进行比较,根据血清差异表达多肽建立弓形虫感染诊断方法,并验证其准确性。结果与健康对照组相比,弓形虫IgG抗体阳性组血清m/z 3 639.4、8 125.51、8 139.24、3 254.51、3 238.39、2 656.91、7 765.07、2 078.03、1 940.3高表达,在m/z 3 954.25、4 269.09、2 750.17、6 447.53、1 735.96、6 645.92、4 280.84低表达,根据差异表达多肽,利用ClinProTool建立的诊断方法对20例弓形虫IgG抗体阳性及15例IgM抗体阳性人群血清进行验证,准确率100%。结论利用血清差异多肽谱结合ClinProTool建立的诊断方法准确率高,有望用于人群弓形虫感染筛查。Objectives To devise a new technique to diagnose Toxoplasma gondii infection in pregnant women and to screen serum markers. Methods Serum samples were collected from pregnant women with toxoplasmosis and healthy controls.Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS)was combined with analysis using ClinProTool to distinguish the IgG-positive group infected with T.gondii and the healthy controls.A diagnostic technique was devised based on the differential expression of peptides in serum,and its accuracy was verified. Results Compared to the healthy control group,the IgG-positive group infected with T.gondii had significantly increased expression of peptides with an m/z of 3,639.4,8,125.51,8,139.24,3,254.51,3,238.39,2,656.91,7,765.07,2,078.03,and 1,940.3but significantly decreased expression of peptides with an m/z of 3,954.25,4,269.09,2,750.17,6,447.53,1,735.96,6,645.92,and 4,280.84.A diagnostic technique based on differential peptide peaks was created using ClinProTool.Its diagnostic accuracy was 100%in 20 Toxoplasma IgG-positive patients and 15IgM-positive patients. Conclusion This diagnostic technique using serum differential peptides combined with ClinProTool is expected to serve as a new technique to screen for T.gondii infection.

关 键 词：弓形虫 磁珠富集 基质辅助激光解吸电离时间飞行质谱 血清多肽 

分 类 号：R382.5[医药卫生—医学寄生虫学]