人类内源性逆转录病毒囊膜蛋白Syncytin互作蛋白质筛选  

Screening of Proteins Interacting with the Envelop Protein Syncytin of Human Endogenous Retroviruses

作　　者：井敏敏 司徒健文 陶瑞 范欣[1,2] 井申荣 孙鹥[1,2] JING Min-min;SITU Jian-wen;TAO Rui;FAN Xin;JING Shen-rong;SUN Yi(Medical Faculty,Kunming University of Scienoe and Technology,Kunming 650500,Yunnan,China;Department of Clinical Laboratory,the First People's Hospital of Yunnan Province,Kunming 650032,Yunnan,China)

机构地区：[1]昆明理工大学医学院,中国云南昆明650500 [2]云南省第一人民医院检验科,中国云南昆明650032

出　　处：《生命科学研究》2018年第6期438-444,共7页Life Science Research

基　　金：国家自然科学基金资助项目(81100377);云南省应用基础研究项目(2013FB203;31060155)

摘　　要：为了筛选与人类内源性逆转录病毒囊膜蛋白syncytin具有相互作用的蛋白质,首先分别构建诱饵质粒Syncytin-pKT25与基因组文库质粒,然后共转入报告菌株DHM1感受态细胞中,并采用抗性筛选得到含有互作蛋白质的菌株。结果发现,筛选到一条包括31个氨基酸的短肽。进一步通过回复杂交和Co-IP验证,确定筛选到的蛋白质与syncytin是否具有相互作用。实验结果表明,筛选到一条与syncytin具有相互作用的氨基酸短肽SJ-1。In order to screen proteins that interact with the envelope protein syncytin of human endogenous retroviruses, the bait plasmid Syncytin-pKT25 and genomic library plasmids were constructed respectively, and they were co-transfected into the competent reporter strain DHM1 cells. After screening for antibiotic resistance, the strains containing the interacting proteins were identified and a short peptide consisting of 31 amino acids was found. The interaction between syncytin and the peptide was verified by recombination and Co-IP validation. The result demonstrated preliminarily that the short peptide named SJ-1 is an interacting peptide with syncytin.

关 键 词：细菌双杂交 互作筛选 SYNCYTIN SJ-1 

分 类 号：Q71[生物学—分子生物学]