二代测序技术在疑难生物检材法医DNA检验的研究进展  

Research Progresses of Next-generation Sequencing Applied in Forensic DNA Analysis of Difficult Specimens

作　　者：程凤 严江伟 CHENG Feng;YAN Jiang-wei(College of Forensic Medicine,Shanxi Medical University,Taiyuan 030001,Shanxi,China;Beijing Institute of Genomics, Chinese Academy of Sciences,Beijing 100029,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]山西医科大学法医学院,中国山西太原030001 [2]中国科学院北京基因组研究所,中国北京100029 [3]中国科学院大学,中国北京100049

出　　处：《生命科学研究》2018年第6期511-516,共6页Life Science Research

基　　金：国家自然科学基金资助项目(81571857);中科院重点部署项目(KGFZD-135-16-021)

摘　　要：测序技术的不断发展使二代测序技术在测序通量提高和读长增加的同时,成本也大幅度降低。目前二代测序技术已广泛应用于生命科学各领域研究中,并且也逐渐受到法医学者的关注。文中主要阐述了二代测序技术在法医学实践中常见的微量、降解及混合等疑难生物检材DNA检验中的研究进展,对其未来的发展进行了展望,并提出了可能面临的挑战。With the development of sequencing technology,next-generation sequencing has been widely applied in the field of life science due to its high throughput,increased reading length and significant cost reduction.It has also gradually attracted the attention of forensic scientists.Here,the advances of next-generation sequencing applied in forensic DNA analysis of difficult specimens were reviewed,and its development and challenges would be faced in the future were described.

关 键 词：法医物证 二代测序技术 微量生物检材 降解生物检材 混合生物检材 

分 类 号：Q503[生物学—生物化学] R89[医药卫生—法医学]