醋酸铅染毒人外周血淋巴细胞诱发XRCC1、hOGG1基因表达变化的研究  

Study on expression changes of XRCC1 and h OGG1 genes induced by lead acetate in human peripheral blood lymphocytes

作　　者：刘祥铨[1] 吴京颖[1] 施文华[1] 吴小南[2] 刘合焜[2] LIU Xiang-quan;WU Jing-ying;SHI Wen-hua(Fuzhou Center for Disease Control and Prevention,Fuzhou 350004,Fujian,China)

机构地区：[1]福州市疾病预防控制中心,福建福州350004 [2]福建医科大学

出　　处：《中国校医》2018年第12期910-912,共3页Chinese Journal of School Doctor

基　　金：福建省卫生系统中青年骨干人才培养项目资助(2014-ZQN-JC-35)

摘　　要：目的研究醋酸铅染毒人外周血淋巴细胞对XRCC1、hOGG1基因表达变化的影响。方法用0、20、40和80μmol/L浓度醋酸铅染毒人外周血淋巴细胞24h,采用qPCR法和Western-Blot法测定XRCC1、hOGG1基因的mRNA和蛋白表达水平。结果与对照组相比,醋酸铅染毒诱导人外周血淋巴细胞XRCC1、hOGG1基因的mRNA和蛋白表达水平随醋酸铅染毒浓度增加而下降,差异有统计学意义(P<0.01)。结论醋酸铅染毒抑制XRCC1、hOGG1基因表达水平,导致细胞对DNA损伤修复能力下降。Objective To study the effect of expression changes of XRCC1 and hOGG1 genes induced by lead acetate in human peripheral blood lymphocytes. Methods Human peripheral blood lymphocytes were exposed to lead acetate at concentrations of 0,20,40, and 80μmol/L for 24h,separately.Then the mRNA and protein expression levels of XRCC1 and hOGG1 genes were determined by qPCR and Western-Blot. Results Compared with the control group, the mRNA and protein expressions of XRCC1 and hOGG1 genes induced by lead acetate were decreased with the increase of lead acetate concentrations in human peripheral blood lymphocytes(all P<0.01). Conclusion The lead acetate exposure inhibits the expressions of XRCC1 and hOGG1 genes, resulting in a decrease of the ability of cells to repair DNA damage.

关 键 词：醋酸铅 人外周血淋巴细胞 XRCC1 HOGG1 

分 类 号：R135.1[医药卫生—劳动卫生]