苯扎氯胺制作的大鼠先天性巨结肠症模型与肠道炎症反应  

Existence of continuous enhancement of intestinal inflammation in rat model of benzalkonium chloride-treated Hirschsprung's disease

作　　者：余辉[1] 潘伟康[1] 郑百俊[1] 谢崇 赵育樱 田东浩 高亚[1] Yu Hui;Pan Weikang;Zheng Baijun;Xie Chong;Zhao Yuying;Tian Donghao;Gao Ya(Department of Pediatric Surgery, Second Affiliated Hospital, Xi’an Jiaotong University, Xi’an 710004, China)

机构地区：[1]西安交通大学第二附属医院小儿外科,710004

出　　处：《中华小儿外科杂志》2018年第12期918-922,共5页Chinese Journal of Pediatric Surgery

基　　金：国家自然科学基金(81741096、81770513、81701501);陕西省科技新星(2019KJXX-048);西安交通大学第二附属医院科技新苗项目 (RC(XM)201703).

摘　　要：目的验证苯扎氯胺制作的先天性巨结肠症动物模型是否存在肠道炎症并探索上述炎症规律。方法采用0.1%-30min苯扎氯胺液包绕SD大鼠肠管制作先天性巨结肠症动物模型。18只SD大鼠遵循随机原则采用随机数字表法平均分为对照组、苯扎氯胺组。分别于术后第7、14、21天采用免疫组织化学检测对照组、苯扎氯胺组肠管C反应蛋白(C-reactive protein,CRP)、肿瘤坏死因子α(turner necrosis factorα,TNF-α)的表达,采用酶联免疫吸附测定对照组、苯扎氯胺组血清CRP、TNF-α表达水平。结果对照组、苯扎氯胺组大鼠在实验期间均存活,未出现大鼠死亡。术后第7、14、21天免疫组织化学结果证实对照组、苯扎氯胺组肠管CRP、TNF-α表达阳性;苯扎氯胺组肠管CRP、TNF-α表达强度自黏膜层向肌层方向均逐渐减弱,且随时间延长逐渐增强。酶联免疫吸附测定术后第7、14、21天苯扎氯胺组血清CRP表达水平(×103ng/ml)分别为1.94±0.31、1.18±0.23和2.45±0.42,对照组CRP表达水平分别为0.21±0.22、0.31±0.24和0.18±0.19,组间各时间点比较,差异均有统计学意义(P<0.05);酶联免疫吸附测定术后第7、14、21天苯扎氯胺组TNF-α表达水平(×102ng/ml)分别为1.92±0.10、2.05±0.12和2.28±0.16,对照组TNF-α表达水平分别为1.74±0.09、1.82±0.07、1.78±0.09,组间各时间点比较,差异均有统计学意义(P<0.05)。结论苯扎氯胺制作先天性巨结肠症动物模型存在肠道炎症。Objective To verify whether intestinal inflammation exists in rat model of benzalkonium chloride-treated Hirschsprung's disease and to explore its regulation mechanism. Methods Serosal application of 0.1%-30 min benzalkonium chloride was applied for establishing a model of Hirschsprung's disease. The expression levels of C-reactive protein (CRP) and tumor necrosis factor-α (TNF-α) were detected by immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) at Day 7, 14 and 21 postoperation respectively. Results All rats survived. The immunohistochemical results showed the positive expressions of CRP and TNF-α in control and animal models at Days 7, 14 and 21 postoperation. Compared with control groups, higher serum level of CRP and TNF-α appeared at Days 7, 14 and 21. The results of ELISA showed that the expression levels of CRP(×103 ng/ml)were 2.45±0.42(day 21), 1.94±0.31 (day 14) and 1.18±0.23 (day 7) (P<0.05) and the expression levels of TNF-α(×102 ng/ml)2.28±0.16(day 21), 2.05±0.12(day 14)and 1.92±0.10(day 7) in BAC groups (P<0.05). All differences were statistically significant. Conclusions Intestinal inflammation exists in rat model of benzalkonium chloride-treated Hirschsprung's disease.

关 键 词：HIRSCHSPRUNG病 动物模型 肠道炎症 炎症因子 

分 类 号：R722.1[医药卫生—儿科] R-332[医药卫生—临床医学]